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首页> 外文会议>Three-Dimensional and multidimensional microscopy: Image acquisition and processing >Measurement of the chirp-dependent fluorescence response: a window to excited-state population dynamics
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Measurement of the chirp-dependent fluorescence response: a window to excited-state population dynamics

机译：测量啁啾依赖性荧光反应：兴奋状态群体动态的窗口

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High intensity chirped pulses can be used for probing microscopic chemical environments through the use of a particular choice of dye, for instance SNAFL2. The basis for this technique is that the excited state populations can be manipulated through control over the temporal order of the excitation frequencies in the excitation pulse - i.e. chirp - - with the outcoming fluorescence as the reporting parameter. A chirp dependent fluorescence response can also be observed in larger molecular systems with more degrees of freedom like for instance green fluorescent proteins. In preparation for application of the technique to microscopy we use a facility permitting observation of this phenomenon in various dyes with high sensitivity. High power, 30 fs pulses from an OPA, tunable from 400 nm to 1.5 micron are used. These pulses with a repetition rate of 1 kHz are sufficiently intense that a relatively large sample region can be excited to saturation from which then a sub-region with uniform excitation conditions can be selected for signal collection.
机译：高强度啁啾脉冲可用于通过使用特定选择染料来探测微观化学环境，例如SNAFL2。这种技术的基础是通过控制激发脉冲 - 即啁啾 - I.Chirp - - 作为报告参数的荧光的励磁频率的时间顺序来操纵激发态群。在较大的分子系统中也可以观察到啁啾依赖性荧光响应，其自由度更高的自由度，例如绿色荧光蛋白。为了准备应用技术的显微镜，我们使用具有高灵敏度的各种染料中这种现象的设施。采用高功率，30 FS脉冲从400nm可调谐到1.5微米。这些具有1kHz的重复率的脉冲足够强烈地强烈地强烈地激发到饱和度到饱和度，然后可以选择具有均匀激励条件的子区域用于信号收集。

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