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首页> 外文会议>Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI >Measurement of the chirp-dependent fluorescence response: a window to excited-state population dynamics
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Measurement of the chirp-dependent fluorescence response: a window to excited-state population dynamics

机译：chi依赖性荧光响应的测量：激发态种群动态的窗口

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Abstract: High intensity chirped pulses can be used for probing microscopic chemical environments through the use of a particular choice of dye, for instance SNAFL2. The basis for this technique is that the excited state populations can be manipulated through control over the temporal order of the excitation frequencies in the excitation pulse - i.e. chirp - - with the outcoming fluorescence as the reporting parameter. A chirp dependent fluorescence response can also be observed in larger molecular systems with more degrees of freedom like for instance green fluorescent proteins. In preparation for application of the technique to microscopy we use a facility permitting observation of this phenomenon in various dyes with high sensitivity. High power, 30 fs pulses from an OPA, tunable from 400 nm to 1.5 micron are used. These pulses with a repetition rate of 1 kHz are sufficiently intense that a relatively large sample region can be excited to saturation from which then a sub-region with uniform excitation conditions can be selected for signal collection. !15
机译：摘要：通过使用特定的染料选择，例如SNAFL2，高强度chi脉冲可用于探测微观化学环境。这项技术的基础是，可以通过控制即将发出的荧光作为报告参数的激发脉冲（即线性调频脉冲）中的激发频率的时间顺序来控制激发态种群。还可以在较大的分子系统中以更高的自由度观察到chi依赖的荧光响应，例如绿色荧光蛋白。在准备将该技术应用于显微镜时，我们使用了一种可以高灵敏度地观察各种染料中这种现象的设备。使用来自OPA的高功率30 fs脉冲，可调范围为400 nm至1.5微米。这些具有1 kHz重复频率的脉冲足够强，可以将一个相对较大的样本区域激发到饱和状态，然后可以从中选择具有均匀激发条件的子区域进行信号收集。 ！15

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