• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Conference on biomedical applications of raman spectroscopy >Detection of angiotensin II binding to single adrenal zona glomerulosa cells by confocal Raman microspectroscopy
【24h】

Detection of angiotensin II binding to single adrenal zona glomerulosa cells by confocal Raman microspectroscopy

机译：通过共焦拉曼微型光谱检测血管紧张素II与单肾上腺Zona肾小球细胞的结合

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We developed a confocal Raman microspectroscopic technique to study ligand-receptor bindings in single cells using Raman-labeled ligands and surface-enhanced Raman scattering (SERS). The adrenal zona glomerulosa (ZG) cells were used as a model in this study. ZG cells have a high density of angiotensin II (AII) receptors on the cellular membrane. There are two identified subtypes of AII receptors,namely AT1 and AT2 receptors. AII is a peptidic hormone, which upon binding to its receptors, stimulates the release of aldosterone from ZG cells. The cellular localization of these receptors subtypes was detected in single ZG cells by using immunocomplexation of receptors with specific antibodies and confocal Raman microspectroscopy. In the binding study, we used biotin-labeled AII to bind to its receptors in ZG cells. Then, avidin and Raman-labeled AII. The binding was measure directly on the single ZG cells. The results showed that the binding was displaced with unlabeled AII and specific AII antagonists. This is a rapid and sensitive technique for detection of cellular ligand bindings as well as antagonists screening in drug discovery.
机译：我们开发了一种使用拉曼标记的配体和表面增强的拉曼散射（SERS）在单细胞中研究单细胞中的配体受体结合的共焦拉曼微型光谱技术。肾上腺ZonaLoMerulosa（Zg）细胞被用作本研究中的模型。 Zg细胞在细胞膜上具有高密度的血管紧张素II（AII）受体。 AII受体存在两种鉴定的亚型，即AT1和AT2受体。 AII是一种肽激素，其在与其受体结合后，刺激来自Zg细胞的醛固酮的释放。通过使用具有特异性抗体和共焦拉曼微痉挛的受体的免疫分解，在单ZG细胞中检测这些受体亚型的细胞定位。在结合研究中，我们使用生物素标记的AII与Zg细胞中的受体结合。然后，逃生素和拉曼标记的AII。将结合直接测量在单个Zg细胞上。结果表明，结合与未标记的AII和特异性AII拮抗剂置换。这是一种快速敏感的方法，用于检测细胞配体束缚以及药物发现中的拮抗剂筛选。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号