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RHIZOCTONIA BLIGHT OF IMPATIENS AND ITS CONTROL

机译:Rhizoctonia枯萎和它的控制

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An outbreak of a foliar disease on New Guinea impatiens (Impatiens hawkeri Bull) was observed in a nursery in Dade County, Florida. Symptoms on leaves were water-soaked spots that increased rapidly in size and became light to dark brown necrotic areas. A Rhizoctonia-like fungus was isolated from infected leaves and stems on corn meal agar subcultured on potato-dextrose agar and identified as Thanatephorus cu-cumeris (Frank) Donk (anamorph Rhizoctonia solani Kuehn). Six plants were randomly inoculatedwith agar blocks containing the isolate. Control plants received agar blocks without the pathogen. Plants were placed in a modified humidity chamber (polyethylene bags) for 48 hours and then transferred to a greenhouse at 27 °C. The symptoms appeared as water soaked spots, 10mm in diameter, that enlarged to 25mm or more and turned dark brown. A mycelial web grew over the leaves, killing them, and spreading from leaf to leaf. Small brown sclerotia and mycelium were found on leaves and stems, typical of that found on nursery plants. Thanatephorus cucumeris was consistently re-isolated from inoculated plants with no symptoms observed on uninoculated plants, fulfilling Koch's postulates.
机译:在佛罗里达州戴德县的苗圃中观察到​​新的豚鼠(Impatiens Hawkeri Bull)的叶面疾病的爆发。叶子上的症状是水浸斑点,速度迅速增加,并成为深棕色坏死区域的光。从受感染的叶片和茎上孤立的rhizoctonia样真菌,玉米粉琼脂在马铃薯右旋糖琼脂上培养,并鉴定为Thathatephorus Cu-Cumeris(Frank)Donk(anomorph Rhizoctonia solani Kuehn)。将六株植物随机接种含有分离物的琼脂嵌段。对照植物接受没有病原体的琼脂块。将植物置于修饰的湿度室(聚乙烯袋)中48小时,然后在27℃下转移至温室。症状出现为水浸泡斑,直径为10毫米,扩大到25mm或更大,变为深棕色。菌丝网在叶子上生长,杀死它们,并从叶子到叶子蔓延。在叶子和茎上发现小棕色肠菌和菌丝体,典型的植物植物中发现。在未征收的植物上始终如一地从接种植物中重新分离出来的植物,无需观察到植物的症状,实现了Koch的假设。

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