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首页> 外文会议>Annual Meeting of the Florida State Horticultural Society >SURVEY OF STEM PITTING CITRUS TRISTEZA VIRUS IN COMMERCIAL CITRUS GROVES IN FLORIDA
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SURVEY OF STEM PITTING CITRUS TRISTEZA VIRUS IN COMMERCIAL CITRUS GROVES IN FLORIDA

机译：佛罗里达州商用柑橘树林中茎烧蚀柑橘Tristeza病毒的调查

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Citrus tristeza virus (CTV) has affected how citrus is grown in Florida since the 1950s. The brown citrus aphid, first detected in Florida in 1995, is an efficient vector of CTV and is capable of spreading severe forms of CTV throughout the state. Theuse of molecular markers for CTV led to the discovery of aphid transmitted stem-pitting forms of CTV (SP-CTV) in Polk County, central Florida. A survey to determine if SP-CTV was present was undertaken for the eleven counties representing 80% of commercial citrus production in Florida. Five sweet orange and two grapefruit sites per county were surveyed using a hierarchical bulk sampling procedure. Immuno-capture reverse transcriptase polymerase chain reaction (IC-RT-PCR) with Type II primers was used for initial screening followed by other SP-CTV markers for positive samples. Fifty-five percent (42 of 77) of the sites surveyed tested negative in all samples. The majority of sites testing positive, 61 %, had a less than a 5% chance of any single tree in that block testing positive. Only six sites had a greater than a 10% chance of any one tree testing positive for the SP-CTV markers. There were two main pattern profiles of markers present. Not all of these isolates have been evaluated in biological indicators so whether they can cause significant damage and what their threat is to the citrus industry has yet to be determined. Currently, a strong Citrus Budwood Registration Program and increasing the number of budwood source trees under protective screen will prevent the spread of severe forms of CTV throughout the nursery industry.
机译：柑橘Tristeza病毒（CTV）影响了自20世纪50年代以来在佛罗里达州种植的柑橘。棕色柑橘蚜虫于1995年在佛罗里达州检测到，是CTV的有效载体，能够在整个状态下传播严重的CTV形式。用CTV的分子标记导致佛罗里达州中部波尔克县的CTV（SP-CTV）的蚜虫透射茎拍态斑点形式的发现。确定SP-CTV是否存在的调查是为佛罗里达州商业柑橘生产的80％的11个县进行了调查。使用分层批量采样程序调查每座甜橙和两个葡萄柚遗址。免疫捕获逆转录酶聚合酶链反应（IC-RT-PCR）用II型引物用于初始筛选，然后是阳性样品的其他SP-CTV标记物。在所有样品中调查的位点五十五（共777个）的遗址。大多数网站测试阳性61％，在该块测试阳性中的任何一棵树都有不到5％的几率。只有六个站点的任何一棵树为SP-CTV标记阳性的几率大于10％。存在标记有两种主要模式概况。并非所有这些分离株都已在生物指标中进行评估，因此它们是否会造成重大损害以及他们的威胁对柑橘行业尚未确定。目前，强大的柑橘德伍德注册计划和增加保护屏幕下的斗伍德源树数量将防止整个幼儿园严重形式的CTV传播。

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