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Fluorescence properties of indocyanin green - part 1: in-vitro study with micelles and liposomes

机译：吲哚菁绿的荧光特性 - 第1部分：用胶束和脂质体的体外研究

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摘要

ICG is a tricarbocyanin fluorescent dye used in angiography. Several reports point out the advantage of ICG to fluoresce in the near IR wavelength range enabling the imaging of deep tissues. If the fluorescence characteristics of ICG in buffer or protein solutions are described in vitro, little is known concerning the physicochemical properties of ICG. This study aims to evaluate the physicochemical and fluorescence spectral characteristics of ICG in aqueous solution and in presence of micelles and liposomes. ICG exhibits a tensioactive property and when incubated with micelles and liposomes tends to be aggregated or embedded at the interface. The fluorescence is very low in the aggregated form and is very high when ICG is embedded at the interface and a shift of the emission peak toward longer wavelength is observed. Such in vitro study could contribute to a better understanding of some observed unusual properties of ICG in vivo.
机译：ICG是用于血管造影的三碳糖蛋白荧光染料。有几个报告指出ICG的优势，在接近IR波长范围内发光，从而实现深层组织的成像。如果在体外描述缓冲液或蛋白质溶液中ICG的荧光特性，则有关ICG的物理化学性质，则少于众所周知。本研究旨在评估ICG在水溶液中的物理化学和荧光光谱特性，并在胶束和脂质体存在下。 ICG表现出张力活性性，并且当与胶束孵育时，脂质体倾向于聚集或嵌入界面。荧光以聚集形式非常低，并且当ICG嵌入界面时非常高，观察到发射峰值朝向较长波长的偏移。这种体外研究可能有助于更好地了解ICG在体内的一些观察到的不寻常性质。

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