首页> 外文会议>Annual Meeting of the United States Animal Health Association >SIGNIFICANCE OF HEAVY FECAL SHEDDING OF MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS (MAP): COMPARISON OF FECAL CULTURE, REAL-TIME AND NESTED PCR TESTING
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SIGNIFICANCE OF HEAVY FECAL SHEDDING OF MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS (MAP): COMPARISON OF FECAL CULTURE, REAL-TIME AND NESTED PCR TESTING

机译:重症粪便分枝杆菌的重要性植分数亚种分枝杆菌(MAP):粪便培养,实时和嵌套PCR测试的比较

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The potential that clumping by Mycobacterium avium subspecies paratuberculosis can influence the quantity of organism identified by fecal culturing was analyzed in a prospective, blinded study using comparative fecal culture, hspX real-time PCR and direct and nested IS1311-based PCR testing. Of the 22 fecal samples identified as coming from "heavy shedders" by fecal culture, only 7 fecal cultures had positive correspondence with realtime and nested PCR. Clumping by Map within fecal samples can causequantitative misrepresentation of the degree of fecal shedding within a given fecal specimen.
机译:通过分枝杆菌贫困的分枝杆菌的潜力可以影响粪族培养的群体鉴定的生物体的数量,并在使用对比粪便培养,HSPX实时PCR和直接和嵌套的基于IS1311的PCR测试中分析了粪便培养的可能性。在由粪便培养中鉴定为来自“重型患病者”的22个粪便样本中,只有7个粪便培养物与实时和嵌套的PCR具有阳性对应。粪便中的地图丛生可以在给定的粪便标本中占据粪便脱落程度的误差。

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