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Mapping QTLs and ESTs associated with drought tolerance in rice towards discovery of candidate genes

机译:将QTL和QTL和EST映射到水稻中的耐旱性,朝向发现候选基因的发现

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The genetic and molecular dissection of stress tolerance has led to the identification of either genomic regions involved in stress tolerance (major loci or QTLs), or DNA sequences known to play a role in molecular stress responses (stress related genes, cis-acting elements and transcription factors). Although QTL analysis and gene cloning have been used to investigate the same stress responses, the relationship between QTLs and stress related sequences is still far from understood and remains a challenge. In the last couple of years, our understanding of the processes underlying plant response to drought, at the molecular and whole-plant levels (rainfed rice ecosystem), has rapidly progressed. The application of molecular markers and genetic linkage maps has allowed specific regions of the genome controlling drought-related polygenic traits, called quantitative trait loci (QTLs), to be identified. Key QTLs have been mapped for root morphology, root distribution, drought avoidance, root penetration ability, osmotic adjustment, dehydration tolerance, stomatal conductance, leaf rolling, heading date, cell membrane stability, abscisic acid accumulation, and several other phenological traits (Price et al, 1999; Katiyar et al., 2003).
机译:胁迫耐受性的遗传和分子解剖导致参与胁迫耐受性(主要基因座或的QTL),或者已知在分子应激反应(胁迫相关基因,顺式作用元件中起作用的DNA序列以及基因组区域的识别转录因子)。虽然QTL分析及基因的克隆已被用于研究相同的应激反应,和的QTL应激相关序列之间的关系还远远没有理解并仍然是一个挑战。在过去的几年中,我们对干旱的植物响应底层,在分子和全株水平(旱作水稻生态系统)的过程的认识,进步很快。分子标记遗传连锁的应用程序映射已经允许基因组的特定区域控制干旱相关基因性状,称为数量性状基因座(QTL),待鉴定。键的QTL已经被映射为根的形态,根系分布,避旱,根穿透能力,渗透调节,脱水耐受性,气孔导度,卷叶,抽穗期,细胞膜的稳定性,脱落酸积累等几个候性状(Price等人,1999; Katiyar等人,2003)。

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