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Biocontrol activity of Pythium oligandrum and Coniothyrium minitans in pelleted and film-coated seed

机译:在颗粒和薄膜涂层种子中的钙寡糖芽孢杆菌和Coniothylium minitans的生物控制活性

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Oospores of Pythium oligandrum, produced in a cane molasses liquid medium, were coated onto cress and sugar-beet seeds by commercial seed pelleting or film-coating procedures. Oospore germination was unaffected by the coating treatments. In glasshouse pot trials, both types of treatment gave significant control of damping-off in cress and sugar beet caused by Pythium ultimum and Aphanomyces cochlioides, respectively. In some cases, the control was equivalent to fungicide drenches or standard seed treatments, but little control was achieved with any treatment when the pathogen inoculum potential in soil was high. Oospores survived in pelleted sugar beet seed for up to 6 years at 8 deg C and retained their biocontrol efficacy against Pythium spp., but only at low pathogen inoculum potentials. To mimic treatment of infested seed lots, conidia of Coniothyrium minitans, were film-coated onto Sclerotinia sclerotiorum-infected sunflower seeds and sclerotia by polymer film-coating, using a fluidised-bedseed system. C. minitans had little effect on seedling disease in tests conducted in a peat-soil mix, but completely suppressed apothecial production of sclerotia when placed in soil. The film-coating process decreased the germination of conidia recovered from sunflower seeds, but after storage for 1 year at 10 deg C, C. minitans still grew from 97 percent of seed. The potential for developing P. oligandrum and C. minitans as commercial seed treatments for biocontrol of seedling diseases is discussed.
机译:通过商业种子造粒或薄膜涂料程序涂覆在甘蔗糖蜜液体中的矿石寡酒植物的OOSPORES涂覆在CRESS和糖甜菜籽上。 Oospore发芽不受涂层处理的影响。在Glasshouse Pot试验中,两种类型的治疗方法分别对由钯ULTimum和Aphanomyces Cochlioides引起的Cress和甜菜中的阻尼抑制。在一些情况下,对照等同于杀真菌剂或标准种子处理,但是当土壤中的病原体潜力高时,通过任何治疗达到较小的对照。 Oospores在8℃下含有长达6年的甘蔗甜菜饲养,并保留了它们对糖分子SPP的生物控制效果。但仅在低病原体接种潜力。为了模仿侵染种子批次的侵染种子批次,使用流化床系统通过聚合物薄膜涂层膜涂覆在肠炎肠道感染的向日葵种子和巩膜上。 C. Minitans对泥炭土混合物中进行的试验中的幼苗疾病对幼苗疾病产生影响,而是在置于土壤中时完全抑制巩膜的药物生产。薄膜涂层过程降低了从葵花籽中回收的分类芽的萌发,但在10℃下储存1年后,C.Minitans仍然来自97%的种子。讨论了开发P. oligandrum和C. minitans作为幼苗疾病的商业种子处理的可能性。

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