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首页> 外文会议>International Symposium of the British Crop Protection Council >Biosensors for field-based detection of plant pathogens and pesticide residue analysis: the state-of-the-art technology as a key tool in Integrated Plant Disease Management
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Biosensors for field-based detection of plant pathogens and pesticide residue analysis: the state-of-the-art technology as a key tool in Integrated Plant Disease Management

机译：基于现场的植物病原体和农药残留分析的生物传感器：最先进的技术作为综合植物疾病管理中的关键工具

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In recent years there has been a rapid increase in the number of diagnostics applications in phytopathology and food chemistry based on biosensors, which can be defined as devices incorporating a biological sensing element connected to a transducer. The most advanced biosensor technology includes live, intact cells as the sensory units. At their current status, cell-based technologies could directly compete with immunoenzymic assays and other immunoanalytical systems. To these promising methods belongs the Bioelectric Recognition Assay (BERA) (Kintzios et al., 2001). This method utilizes the natural response of cells to pathogens or other toxic factors. Alternatively, it can utilize so-called membrane-engineered cells, which are cells with tens of thousands of target-specific receptor molecules artificially inserted on the cell surface. In the following we describe, as a representative example, the application of BERA biosensors for the detection of (1) a plant virus (Cucumber mosaic virus ?CMV) and (2) a pesticide (metamidophos) in plant tissues. Furthermore, we present the profile of a novel analytical lab, 3QLabs, which employs biosensors as an integral BMP tool for vegetable and fruit production at a country-wide scale.
机译：近年来，基于生物传感器的植物病理学和食品化学的诊断应用的数量迅速增加，可以定义为包含连接到换能器的生物传感元件的装置。最先进的生物传感器技术包括Live，完整的细胞作为感官单位。在他们目前的状态下，基于细胞的技术可以直接与免疫酶测定和其他免疫分析系统竞争。对于这些有希望的方法属于生物电识别测定（BERA）（Kintzios等，2001）。该方法利用细胞的自然反应对病原体或其他毒性因素。或者，它可以利用所谓的膜工程细胞，其是具有成千上万的靶特异性受体分子的细胞，其在细胞表面上是人工的。在下文中，我们将Bera生物传感器的应用用于检测（1）植物病毒（黄瓜马赛克病毒αCMV）和（2）在植物组织中的杀虫剂（Metamidophos）的应用。此外，我们介绍了一种新的分析实验室，3Qβ的简介，其使用生物传感器作为以全国范围的蔬菜和水果生产的整体BMP工具。

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