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Functionalization of Cowpea Chlorotic Mottle Virus Capsids using Molecular Biology techniques

机译：用分子生物学技术对豇豆氯化斑块病毒衣壳的功能化

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In recent years it has been shown that biological structures can be used as versatile platforms in the field of nanoscience. Our research focuses on the icosahedral plant virus Cowpea Chlorotic Mottle Virus (CCMV), which consists of an RNA genome enclosed by a 28 nm protein capsid. This virus can reversibly be assembled from its protein dimers, even when no RNA is present. We have shown that the empty capsid can be used to encapsulate enzymes and by using confocal microscopy the activity of these enzymes was studied at the single-enzyme level. Another example of the use of CCMV capsids is the synthesis of highly monodisperse Prussian blue nanoparticles. Prussian blue precursor compounds were encapsulated in the capsid, and the nanoparticles were subsequently synthesized by irradiation with UV light. Analysis of the resulting product revealed the presence of very robust capsids containing 18 nm Prussian blue particles, which could be spread in hexagonal patterns on a mica surface.
机译：近年来，已经表明，生物结构可以用作纳米科学领域的多功能平台。我们的研究侧重于IcosaheDral植物病毒豇豆氯化斑块病毒（CCMV），其由由28nm蛋白质衣壳包围的RNA基因组组成。即使没有RNA存在，该病毒也可以从其蛋白质二聚体可逆地组装。我们已经表明，空衣壳可用于包封酶，并通过使用共聚焦显微镜检查这些酶的活性在单酶水平上研究了这些酶的活性。使用CCMV衣壳的另一个例子是高度单分散普鲁士蓝纳米颗粒的合成。普鲁士蓝前体化合物包封在衣壳中，随后通过用UV光照射合成纳米颗粒。所得产物的分析显示出含有18nm普鲁士蓝颗粒的非常强大的衣壳的存在，其可以在云母表面上以六边形图案展开。

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