首页> 外文会议>Beltwide Cotton Conference >DIFFERENTIAL RATE OF RESISTANCE DEVELOPMENT TO BT CRY1AC IN COTTON BOLLWORM, HELICOVERPA ZEA (BODDIE) WHEN SELECTED USING MVP II AND ACTIVATED TOXIN
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DIFFERENTIAL RATE OF RESISTANCE DEVELOPMENT TO BT CRY1AC IN COTTON BOLLWORM, HELICOVERPA ZEA (BODDIE) WHEN SELECTED USING MVP II AND ACTIVATED TOXIN

机译:使用MVP II选择和活化毒素选择时,抗抗棉铃虫抗性发育对BT Cry1Ac的差异速率,Helicoverpa(Boddie)

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摘要

A susceptible laboratory strain of cotton bollworm, Helicoverpa zea, was established from a Monsanto laboratory colony. The baseline susceptibility of this strain to MVP II and Cry1Ac toxin was 24 μg/g and 9μg/g diet, respectively. Subsequently, twoCry1Ac-resistant strains of cotton bollworm were selected using MVP II (MR) or activated Cry1Ac toxin (AR). Larvae that molted into second instar within seven days of selection were reared until pupation on regular diet (containing no Cry1Ac). Current resistant ratios for MR and AR strains are 12.6 and 35.9 fold after seven generations of selection, respectively. Selection studies indicated approximately three times quicker resistance development in the AR compared to the MR strain. Additionally, there were higher fitness costs in terms of fertility and fecundity in the MR compared to the AR strain.
机译:从孟山脉菌落建立了一种易感实验室棉铃虫植物菌菌。该菌株对MVP II和Cry1Ac毒素的基线易感性分别为24μg/ g和9μg/ g饮食。随后,使用MVP II(MR)或活化的Cry1Ac Toxin(Ar)选择TwoCry1Ac抗性棉花菌株。幼虫在选择七天内迁入第二瞬间,直至常规饮食(不含Cry1Ac)进行蛹化。在七代选择后,MR和Ar菌株的电流抗性比例分别为12.6和35.9倍。选择研究表明,与MR菌株相比,AR中的耐受性快速发育大约三倍。另外,与AR菌株相比,在MR的生育率和繁殖力方面存在较高的健康成本。

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