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首页> 外文会议>International Symposium on Ganoderma Research >Regulation of Ganoderma lucidum Polysaccharides on Cytotoxic T Lymphocytes Induced by Dendritic Cells in vitro
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Regulation of Ganoderma lucidum Polysaccharides on Cytotoxic T Lymphocytes Induced by Dendritic Cells in vitro

机译：在体外树突细胞诱导的细胞毒性T淋巴细胞上的Ganoderma lucidum多糖的调节

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This research studied the regulatory effects of Ganoderma lucidum polysaccharides (GL-PS) on cytotoxicity and mechanism of specific cytotoxic T lymphocytes (CTL) induced by dendritic cells (DC) in vitro during the stage of antigen presentation. Cultured murine bone marrow-derived DC were pulsed with P815 tumor cell lysates and co-incubated with or without various concentrations of GL-PS (0.8, 3.2, or 12.8mg·L<'-1>) at the same time. P815 specific CTL were induced by spleen lymphocytes stimulated with mature DC. Non-adherent cells and culture supernatants were harvested on d 5 for analysis of specific cytotoxicity with lactate dehydrogenase (LDH) activity assay, mRNA expression of IFN-γ, granzyme B with RT-PCR assay, and protein expression of IFN-γ, granzyme B with ELISA or western blot assay, respectively. Three concentrations of GL-PS could promote LDH activities released into culture supernatants (P <0.01). It also increased mRNA expression of IFN-γ in CTL (GL-PS 12.8mg·L<'-1> vs RPMI medium 1640, P<0.05) and granzyme B in CTL (P<0.01). Protein production of IFN-γ in culture supernatants (P<0.05) and protein expression of granzyme B in CTL (GL-PS 12.8mg·L<'-1> vs RPMI medium 1640, P<0.05) were also augmented by GL-PS. GL-PS was shown to promote the cytotoxicity of specific CTL induced by DC which pulsed with P815 tumor antigen during the stage of antigen presentation, and the mechanisms of cytotoxicity was believed to be going through IFN-γ and granzyme B pathways.
机译：该研究研究了灵芝多糖（GL-PS）对抗原呈现阶段的树突状细胞（DC）在体外诱导的特异性细胞毒性T淋巴细胞（CTL）的细胞毒性和机理的调节作用。用P815肿瘤细胞裂解物脉冲培养的鼠骨髓衍生的DC，同时与或没有各种浓度的GL-PS（0.8,3.2，或12.8mg·1>）共孵育。 P815特异性CTL被脾脏淋巴细胞诱导，脾脏淋巴细胞刺激，刺激成熟DC。在D 5上收获非粘附细胞和培养上清液，用于分析具有乳酸脱氢酶（LDH）活性测定的特异性细胞毒性，IFN-γ，Granzyme B的mRNA表达，RT-PCR测定，IFN-γ，Granzyme的蛋白表达B分别具有ELISA或Western印迹测定。三种浓度的GL-PS可以促进释放到培养物上清液的LDH活性（P <0.01）。它在CTL（GL-PS 12.8mg·L 1> Vs RPMI培养基1640，P <0.05）中的IFN-γ的MRNA表达增加（P <0.01）。 CTL培养上清液中IFN-γ的IFN-γ的蛋白质产生和CTL的蛋白表达（GL-PS 12.8mg·L < - 1> Vs RPMI培养基1640，P <0.05）也被GL- PS。显示GL-PS促进通过DC诱导的特异性CTL的细胞毒性，所述DC在抗原呈现的阶段脉冲P815肿瘤抗原，并且认为细胞毒性的机制通过IFN-γ和Granzzyme B途径。

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