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首页> 外文会议>Society of Photo-Optical Instrumentation Engineers Conference on Microarrays and Combinatorial Technologies for Biomedical Applications :Design, Fabrication, and Analysis >Automated high-throughput purification of genomic DNA from whole blood using Promega's MagneSil~(TM) paramagnetic particles with either the Max Yield or MagneSil~(TM) ONE normalized purification methods
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Automated high-throughput purification of genomic DNA from whole blood using Promega's MagneSil~(TM) paramagnetic particles with either the Max Yield or MagneSil~(TM) ONE normalized purification methods

机译：使用Promega的镁〜（TM）顺磁颗粒从全血的自动化高通量纯化，MAX产量或磁头杆菌〜（TM）一种标准化纯化方法

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摘要

Two different methods of automated high throughput purification of genomic DNA from human whole blood in 96 well plates are described. One method uses MagneSil~(TM) paramagnetic particles to purify a maximal amount of the DNA present in the sample. Another method, the Magnesil~(TM) ONE system, allows for the purification of a predetermined amount of DNA from human whole blood. Protocols for the purification of 100 ng or, alternatively 1 ug, of human genomic DNA from whole blood using MagneSil~(TM) paramagnetic particles and a Beckman BioMek~(TM) FX robot are described. With the maximal yield purification system, typical DNA yields fall in the range of 4-9 ug of DNA from 200ul of human whole blood, depending upon the white cell content of donor sample. For situations where DNA achiving is desired, or when the number of downstream sample applications is not clearly defined (e.g. multiple SNP analyses) the maximal yield method is usually preferred. However, in situations with a defined downstream application (e.g. criminal databasing or use of a defined set of amplifications) where purifying DNA in a narrow concentrate range streamlines the high throughput purification and analysis process, the automated MagneSil~(TM) ONE purification system is the method of choice. DNA from either method is suitable for applications such as PCR, STR, READITTM SNP analysis, and multiplexed PCR systems such as Promega's Y-chromosome deletion detection system.
机译：描述了来自96个孔板中的人类全血的基因组DNA的两种不同的自动化高通量净化方法。一种方法使用氧化镁〜（TM）顺磁颗粒纯化样品中存在的最大量的DNA。另一种方法，氧化镁〜（TM）一个系统，允许从人类全血中纯化预定量的DNA。描述了100ng纯化的10ng或或者1，或者1ug，使用麦尾然血管〜（tm）顺磁颗粒和Beckman Biomek〜（TM）FX机器人的人类基因组DNA的纯化。通过最大产率净化系统，根据供体样品的白色细胞含量，典型的DNA产量来自人类全血的4-9ug DNA的范围。对于需要DNA实现的情况，或者当未明确定义下游样品应用的数量时（例如，多个SNP分析）通常优选最大产量法。然而，在具有定义下游应用的情况（例如犯罪数据库或使用定义的扩增集）的情况下，其中纯化浓缩物范围中的DNA精简高通量净化和分析过程，自动氧化镁〜（TM）一个净化系统是选择方法。来自任一方法的DNA适用于PCR，STR，Readittm SNP分析和多重PCR系统，例如Promega的Y-染色体缺失检测系统。

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