CRYOPRESERVATION OF ATLANTIC SALMON (SALMO SALAR L.) SEMEN AND SURVIVAL RATE OF PROGENY

机译：大西洋三文鱼（沙摩酱L.）精液和后代生存率的冷冻保存

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The cryopreservation effect is usually measured by percentage pf motile spermatozoa after freezing/thawing procedure but these is not always reflected in fertilization success. The best method for verification of efficiency of cryopreservation biotechniques is hatching rate determination after fertilization with freezing sperm (Glogowski, et al. 2002). It has been estimated that dimethylsulphoxide (DMSO) commonly used cryoprotectant is hydroxyl-free radical producer (Miller and Cornwell, 1978) and during cryopreservation harms the DNA which results in lethality during the embryo development. On the other hand, Horvath et al. (2000) proved that methanol (MeOH) used as cryoprotectant affects motility worse than DMSO, but results in good biological effect shown in hatching. The aim of this experiment was optimalization of cryopreservation biotechnique of Atlantic salmon sperm by testing eight variants of cryoconservation. The testing factors were: cryoprotectants (DMSO and MeOH), extenders (glucose and sucrose) and technique of freezing (pellets and straws).

机译：冷冻保存效果通常通过冻结/解冻程序后PF电机精子的百分比测量，但这些并不总是反映在施肥成功中。用于验证冷冻保存生物技术效率的最佳方法是用冷冻精子施肥后的孵化率测定（Glogowski，等人。2002）。据估计，二甲基磺氧化物（DMSO）常用的冷冻保护剂是无羟基的自由基生产者（MILLER和CORNWELL，1978），并且在冷冻保存期间损害DNA，导致胚胎发育过程中的致死性。另一方面，Horvath等人。（2000）证明，用作冷冻保护剂的甲醇（MeOH）影响比DMSO更差的运动，但导致孵化中显示的良好生物学效果。该实验的目的是通过测试八种粘性的低温变种来对大西洋鲑鱼精子进行冷冻保存生物技术的最佳化。测试因素是：冷冻保护剂（DMSO和MeOH），扩展剂（葡萄糖和蔗糖）和冷冻（颗粒和吸管）的技术。

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《International Conference of the European Aquaculture Society》|2008年||共2页
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K. Dziewulska; A. Rzemieniecki; J. Domagala; J. Glogowski;
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CRYOPRESERVATION OF ATLANTIC SALMON (SALMO SALAR L.) SEMEN AND SURVIVAL RATE OF PROGENY

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