首页> 外文会议>International Conference of the European Aquaculture Society >CELL CYCLE SYNCHRONIZATION OF GOLDFISH (CARASSIUS AURATUS) CATJDAL FIN DERIVED FIBROBLASTS WITH DIFFERENT CULTURE rnNDlTIONS TO OBTAIN GO/G1 PHASE SOMATIC CELL POPULATION FOR FISH NUCLEAR TRANSFER

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CELL CYCLE SYNCHRONIZATION OF GOLDFISH (CARASSIUS AURATUS) CATJDAL FIN DERIVED FIBROBLASTS WITH DIFFERENT CULTURE rnNDlTIONS TO OBTAIN GO/G1 PHASE SOMATIC CELL POPULATION FOR FISH NUCLEAR TRANSFER

机译：金鱼（Carassius auratus）Catjdal Fin衍生成纤维细胞的细胞周期同步，具有不同培养的rnndltions，以获得用于鱼核转移的Go / g1相体细胞群

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One of the most important factors that determine the success of the development of cloned embryo is the cell cycle stage of the cloning donor cells (Campbell et al., 1999; Gibbons et al., 2002). Cell cycle synchronization studies from somatic cells inmammals have been reported, however, no work has been done on the control of the cell cycle stages in fish somatic cells. The aim of this study was to assess by flow cytometry the cell cycle of goldfish caudal fin derived fibroblast under different culture conditions namely, culture to confluence, serum starvation and roscovitine treatment. The efficiency of roscovitine at different concentrations in arresting cells at GO/G1 phase was also determined. In addition, we examined the apoptotic effect of different culture conditions on goldfish cells.

机译：确定克隆胚胎的发展成功的最重要因素之一是克隆供体细胞的细胞周期阶段（Campbell等，1999; Gibbons等，2002）。然而，已经报道了来自体细胞哺乳动物的细胞周期同步研究，但没有在鱼体细胞中的控制细胞周期阶段进行工作。本研究的目的是通过流式细胞术评估金鱼尾鳍衍生成纤维细胞在不同培养条件下的细胞周期即，融合融合，血清饥饿和roscovitine治疗。还确定了在GO / G1相的抑制细胞中不同浓度的Roscovitine的效率。此外，我们检查了不同培养条件对金鱼细胞的凋亡效应。

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《International Conference of the European Aquaculture Society》|2008年||共2页
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C.H. Choresca Jr.; S. C. Park; B.C. Lee; O.J. Koo; O.K. Gomez; H.J. Oh; J.H. Kim; J.E. Han; S.P. Shin;
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1. Different culture conditions used for arresting the G0/G1 phase of the cell cycle in goldfish (Carassius auratus) caudal fin-derived fibroblasts. [J] . Choresca-CH Jr, Koo OJ, Oh HJ, Cell biology international. . 2009,第1期

机译：在金鱼（Car鱼）尾鳍衍生的成纤维细胞中，用于阻止细胞周期的G0 / G1期的不同培养条件。
2. Effect of dimethyl sulfoxide on cell cycle synchronization of goldfish caudal fin derived fibroblasts cells. [J] . Reproduction in Domestic Animals . 2010,第5期

机译：二甲基亚砜对金鱼尾鳍衍生成纤维细胞细胞周期同步的影响。
3. Examination of a modified cell cycle synchronization method and bovine nuclear transfer using synchronized early G1 phase fibroblast cells [J] . Urakawa M, Ideta A, Sawada T, Theriogenology . 2004,第3a4期

机译：使用同步的早期G1期成纤维细胞检查改良的细胞周期同步方法和牛核转移
4. CELL CYCLE SYNCHRONIZATION OF GOLDFISH (CARASSIUS AURATUS) CATJDAL FIN DERIVED FIBROBLASTS WITH DIFFERENT CULTURE rnNDlTIONS TO OBTAIN GO/G1 PHASE SOMATIC CELL POPULATION FOR FISH NUCLEAR TRANSFER [C] . C.H. Choresca Jr., S. C. Park, B.C. Lee, International Conference of the European Aquaculture Society . 2008

机译：金鱼（Carassius auratus）Catjdal Fin衍生成纤维细胞的细胞周期同步，具有不同培养的rnndltions，以获得用于鱼核转移的Go / g1相体细胞群
5. Nuclear-cytoplasmic interactions in rat oocytes and reconstructed eggs derived by somatic cell nuclear transfer. [D] . Yoo, Jae Gyu. 2007

机译：大鼠卵母细胞和通过体细胞核转移获得的重建卵中的核质相互作用。
6. Nuclear import of Xenopus egg extract components into cultured cells for reprogramming purposes: a case study on goldfish fin cells [O] . Nathalie Chênais, Thierry Lorca, Nathalie Morin, -1

机译：非洲爪蟾卵提取物成分向培养细胞中的核输入以进行重编程：以金鱼鳍细胞为例
7. Peer Review #4 of "Establishment and characterization of fantail goldfish fin (FtGF) cell line from goldfish, Carassius auratus for in vitro propagation of Cyprinid herpes virus-2 (CyHV-2) (v0.2)" [O] . 2020

机译：同行评论“芬芳金鱼鳍（FTGF）Cell系列来自金鱼，Carassius Auratus的”建立和表征的“Carassius Auratus的体外繁殖”（Cyhv-2）（V0.2））“

CELL CYCLE SYNCHRONIZATION OF GOLDFISH (CARASSIUS AURATUS) CATJDAL FIN DERIVED FIBROBLASTS WITH DIFFERENT CULTURE rnNDlTIONS TO OBTAIN GO/G1 PHASE SOMATIC CELL POPULATION FOR FISH NUCLEAR TRANSFER

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