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首页> 外文会议>International Workshop on Gluten Proteins >CHARACTERIZATION OF OMEGA GLIADINS ENCODED ON CHROMOSOME 1A AND EVIDENCE FOR POST-TRANSLATIONAL CLEAVAGE OF OMEGA GLIADINS BY AN ASPARAGINYL ENDOPROTEASE
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CHARACTERIZATION OF OMEGA GLIADINS ENCODED ON CHROMOSOME 1A AND EVIDENCE FOR POST-TRANSLATIONAL CLEAVAGE OF OMEGA GLIADINS BY AN ASPARAGINYL ENDOPROTEASE

机译：在染色体1A上编码的ωGliadins的表征及芦笋内氨基乙酰吡啶基甘氨酸巨蛋白的翻译裂解的证据

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Three types of wheat ra-gliadins were identified previously, based on N-terminal sequences. Types beginning ARQ or RQ were separated from types beginning KEL by acid PAGE and ion exchange chromatography and referred to as co-2 and co-1 types, respectively1'2. N-terminal sequences of ARQ and KEL types were similar except for the first 8 amino acids of the ARQ type. ARQ and KEL types were associated with chromosomes 1A and ID of bread wheat. A third type beginning SRL was associated with chromosome IB, and referred to as (0-5 type. Since it was not clear how to distinguish between the co-gliadins from chromosomes 1A and ID based on protein or gene sequence data, co-gliadins were purified from T. urartu, the probable donor of the A-genome', and compared with 1A co-gliadins that were purified from the bread wheats Buile86 and Chinese Spring nullisomic ID tctrasomic 1A (CS N1DT1A). N-terminal sequences and masses of the purified co-gliadins suggested that the KEL types (co-l) are derived from ARQ types (co-2) by cleavage between an asparagine and a lysine. Data presented elsewhere indicate that 1A and ID co-gliadins can be separated by HPLC and that 1A co-gliadins are generally somewhat smaller that ID co-gliadins but are similar in terms of N-terminal sequence.
机译：三种类型的小麦RA-麦醇溶蛋白的先前确定的，基于N-末端序列。开始ARQ或RQ类型是从由酸PAGE和离子交换层析开始KEL类型分离，并称为共-2和CO-1的类型，respectively1'2。 ARQ和KEL类型的N末端序列是除了ARQ类型的前8个氨基酸类似。 ARQ和KEL类型用染色体1A和面包小麦的ID相关联。开始SRL第三种类型与染色体IB相关联，并且被称为（0-5型。由于这是不清楚如何基于蛋白质或基因序列数据，共麦醇溶蛋白的从染色体1A和ID共麦醇溶蛋白之间进行区分从T.乌拉尔图中，A基因组”的可能供体，进行纯化，并与来自面包小麦Buile86和中国春缺体ID tctrasomic 1A（CS N1DT1A）。N-末端序列和群众纯化即1A共麦醇溶蛋白相比建议的纯化共麦醇溶蛋白，所述KEL类型（共-1-）从ARQ类型（共-2）由天冬酰胺和赖氨酸之间裂解衍生的。数据呈现别处表明1A和ID共同醇溶蛋白可以通过分离HPLC和1A共同醇溶蛋白一般是ID共醇溶蛋白稍小但在N末端序列的方面类似。

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