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首页> 外文会议>American Chemical Society National Meeting >Biobleaching Efficiency of Aspergillus oryzae Xylanases Produced by Solid Substrate Fermentation
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Biobleaching Efficiency of Aspergillus oryzae Xylanases Produced by Solid Substrate Fermentation

机译：通过固体基质发酵产生的曲霉属植物的生物漂白效率

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The direct use of substrate specific enzymes produced by solid substrate fermentation (SSF) without a downstream processing represents an innovative method for modification of pulp and paper properties and reduction of chemical consumption during bleaching that can bring about positive economical and environmental benefits to the industry. Screening of a number of Aspergillus oryzae isolates on oxygen delignified soda-aq pulp of Eucalyptus grandis as carbon source and enzyme inducer yielded xylanase activities for the best six strains in the range of 500 - 4300 IU/g dry material (DM) with low levels (<1.0 FPU/g DM) of simultaneously produced cellulase. The in situ produced enzymes (whole SSF culture) were used in pulp bleaching without a prior downstream processing of xylanase. The brightness gain over control was enzyme dose dependent and varied between 0.7 and 1.4 points. The optimum moisture content (83%), initial pH (8.3) and time course (4 d) of SSF were determined for the best-performing strain in the bleaching studies (A. oryzae NRRL 3485). Addition of corn steep liquor, an inexpensive nitrogen source, enhanced the xylanase yield considerably. The pH and temperature optima of the xylanase complex of A. oryzae NRRL 3485 was found to be 6.5 and 65°C, respectively. An improved thermal stability was observed at 50°C and pH 6.5 in the presence of eucalyptus pulp substrate. When used at equal enzyme costs and charges in biobleaching of eucalyptus soda-aq pulp, the A. oryzae enzyme outperformed a commercial xylanase control in its efficiency to improve pulp brightness.
机译：通过固体基质发酵（SSF）产生的底物特异性酶而没有下游加工的直接用途代表了改进纸浆和纸张性能的创新方法，并在漂白期间降低化学品消耗，这可以为行业带来积极的经济和环境效益。筛选许多玉米葡萄牙葡萄牙菌的氧化钇血液中的玉米菌蛋白酶和酶诱导剂作为碳源和酶诱导剂的氧化渣，得到了六个菌株的最佳六种菌株（DM）的最佳六种菌株，具有低水平（<0.0fpu / g dm）同时产生的纤维素酶。原位产生的酶（整个SSF培养物）用于纸浆漂白，而无需含木聚糖酶的前下游加工。对照的亮度增益是酶剂量依赖性和变化在0.7和1.4点之间。测定SSF的最佳水分含量（83％），初始pH（8.3）和时间过程（4d）在漂白研究中的最佳菌株（A. Oryzae NRRL 3485）中。添加玉米陡液，廉价的氮源，显着增强了木聚糖酶产率。发现A. oryzae NRRL 3485的木聚糖酶复合物的pH和温度优值分别为6.5和65℃。在桉树浆基材存在下在50℃和pH6.5处观察到改善的热稳定性。当以等于酶成本和Eucalyptus钠-AQ纸浆的生物漂白剂的用量使用时，A. Oryzae酶在其效率上表现出商业木聚糖酶对照，以改善纸浆亮度。

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