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首页> 外文会议>East Asian Chonference on Chemical Sensors >Application of Microarray-Based Method for Functional SNP Genotyping in the Risk of Gastric Carcinoma in Chinese Population
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Application of Microarray-Based Method for Functional SNP Genotyping in the Risk of Gastric Carcinoma in Chinese Population

机译：微阵列为基础族态血管基因分型在中国人群胃癌风险中的应用

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High-throughput SNP detection microarrays were used here to explore the relationship between 5, 10-methylenetetrahydrofolate reductase (MTHFR) gene polymorphism C677T and the risk of gastric carcinoma among population in Jiangsu region, by genotyping the specimens from 170 patients with gastric carcinoma and 140 age- and sex-matched control subjects. PCR products were spotted onto a 3-aminopropyltriethoxysilane coated glass slide to fabricate a microarray, then interrogated by hybridization with dual-color probes (Cy3, Cy5) to determine the SNP genotype of each sample, and the relation between the genotypes and the risk of gastric carcinoma was analyzed. The frequencies of C677T genotype were CC(47.9%), CT(40%), CT(12.1%) in control group and CC(35.9%), CT(45.9%), TT(18.2%) in gastric carcinoma group, respectively. The individuals with 677CT+TT genotype group or 677TT had a 1.67-fold (95% CI: 1.06-2.64) or 2.67-fold (95% CI: 1.382-5.341) increased risk to develop gastric carcinoma compared with those having 677CC genotype. It was shown that the single nucleotide polymorphisms in the MTHFR gene are associated with the risk of gastric carcinoma in the Chinese population.
机译：这里使用高通量SNP检测微阵列来探讨5,10-亚甲基四乙烯酸还原酶（MTHFR）基因多态性C677T与江苏地区人口中胃癌的风险，通过从170例胃癌和140患者进行基因分型来探讨江苏地区人口中的胃癌的风险年龄和性别匹配的控制受试者。将PCR产物被发现到3-氨基丙基三乙氧基硅烷涂覆的玻璃载玻片上以制造微阵列，然后通过用双色探针（CY3，CY5）杂交来询问每个样品的SNP基因型，以及基因型之间的关系和基因型之间的关系及其风险分析胃癌。 C677T基因型的频率分别是对照组的CC（47.9％），CT（40％），CT（12.1％），CC（35.9％），CT（45.9％），CT（45.9％），TT（18.2％），胃癌基团。具有677Ct + TT基因型组或677TT的个体具有1.67倍（95％CI：1.06-2.64）或2.67倍（95％CI：1.382-5.341），与具有677cc基因型的人相比，将胃癌的风险增加。结果表明，MTHFR基因中的单核苷酸多态性与中国人群胃癌的风险有关。

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