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首页> 外文会议>International Epizootic Office/Food and Agriculture Organization fo the United Nations International Scientific Conference on Avian Influenza >Ecology and Molecular Epidemiology of H9N2 Avian Influenza Viruses Isolated in Israel during 2000-2004 Epizootic
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Ecology and Molecular Epidemiology of H9N2 Avian Influenza Viruses Isolated in Israel during 2000-2004 Epizootic

机译：2000-2004截至以色列中孤立的H9N2禽流感病毒生态学和分子流行病学

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摘要

The first two isolates of H9N2 influenza virus were picked up from turkey and chicken hosts in May 2000, but the actual epizootic of the low pathogenicity avian influenza (LPAI) H9N2 virus started in December 2001, following a 1.5-year period of silence, during which the H10N7 and H6N3 influenza viruses were isolated sporadically. The outbreak of the H9N2 influenza began in northern Israel, from where the epizootic spread all over the country. Damage was relatively limited because of the widespread use of an inactivated vaccine. Single isolates were recorded in commercial ostrich and goose flocks, and in a wild pigeon. Apart from the routine serological tests, the diagnostics used the RT-PCR (reverse transcription polymerase chain reaction) test with type-specific primers related to the M and nucleoprotein (NP) genes, and a set of subtype-specific primers related to all the haemagglutinin (HA) and neuraminidase (NA) subtypes. All the primers were specially constructed. The part coding for N-terminus of the H chain of the HA gene of 61 out of 400 isolates was sequenced. The isolates showed a high rate of mutability, and differed distinctly from the H9 prototype strain; they belong to the same phylogenetic lineage divided into three sublin-eages, one of which exhibited a unique cleavage-site motif RSKR. The result indicates that two parallel evolutionary trends originated from the same local" prototype" isolate.
机译：从2000年5月从土耳其和鸡宿主捡起H9N2流感病毒的前两种分离株，但在1.5年的沉默期间，低致病性禽流感（LPAI）H9N2病毒的实际癫痫发作症始于2001年12月，期间H10N7和H6N3流感病毒散发出来。 H9N2流感的爆发始于以色列北部，从外膜展开的地方遍布全国。由于灭活疫苗的广泛使用，损伤相对有限。单独的分离株被记录在商业鸵鸟和鹅羊群中，并在野生鸽子中记录。除了常规血清学试验外，诊断使用与与M和核蛋白（NP）基因相关的类型特异性引物进行RT-PCR（逆转录聚合酶链反应），以及与所有相关的一组亚型特异性引物。 Haemagglutinin（HA）和神经氨酸酶（NA）亚型。所有引物都是专门建造的。测序400个分离株中的HA基因的H链的N-末端的部件进行测序。分离物显示出高度的可变性，与H9原型菌株明显不同;它们属于不同的系统发育谱系分为三个宿林 - 屋，其中一个展示了独特的切割现场MOTIF RSKR。结果表明，来自同一本地“原型”隔离的两个平行进化趋势。

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