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首页> 外文会议>International Symposium on Energy and Protein Metabolism and Nutrition >The heat-induced production of reactive oxygen species regulates protein content in cultured chick skeletal muscle cells
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The heat-induced production of reactive oxygen species regulates protein content in cultured chick skeletal muscle cells

机译：热诱导的反应性氧物种生产调节培养的雏鸡骨骼肌细胞中的蛋白质含量

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Heat stress is a major factor affecting meat production in the poultry industry, especially in hotter regions of the world. A previous in vivo study by our group highlighted that acute heat stress stimulates mitochondrial reactive oxygen species (ROS)production in the skeletal muscle of broiler chickens. Recently, we further clarified that the overproduction of mitochondrial ROS resulted from an increase in the mitochondrial membrane potential, probably due to an increase in substrate oxidation (Kikusato et al, 2010) and a decrease in avian uncoupling protein (avUCP) content (Mujahid et al., 2006). Furthermore, it was reported that heat stress increases protein oxidation and myofibrillar proteolysis in chick cultured skeletal muscle cells (Nakashima et al, 2004). Considering the fact that ROS play a pivotal role in muscle proteolysis in sepsis (Supinski and Callahan, 2007), it could be postulated that heat treatment stimulates mitochondrial ROS production, thereby leading to an increase in skeletal muscle proteolysis. To verify this possibility, we have investigated the effect of heat stress on ROS production, protein content, and the ROS-related expression of genes for avUCP, heme oxygenase-1 (HO-1) andNADPH oxidase 4 (NOX4) in chick skeletal muscle cells in primary culture. We also examine the reducing effects of 4-hydroxy-TEMPO (Tempol), which is a strong antioxidant, on these parameters.
机译：热应力是影响家禽业中肉类生产的主要因素，特别是在世界上更热的地区。我们本集团的先前在体内研究突出显示急性热应激刺激肉鸡骨骼肌肉中的线粒体活性氧物质（ROS）生产。最近，我们进一步阐明了线粒体RO的过度生产是由于线粒体膜电位的增加导致的，可能是由于底物氧化（Kikusato等，2010）的增加和禽类未偶联蛋白（AVUCP）含量的降低（Mujahid Et Al。，2006）。此外，据报道，热应激增加了小鸡培养骨骼肌细胞中的蛋白质氧化和肌原纤维蛋白水解（Nakashima等，2004）。考虑到ROS在脓毒症肌蛋白溶解中发挥关键作用（Supinski和Callahan，2007），可以假设热处理刺激线粒体ROS产生，从而导致骨骼肌蛋白质的增加。为了验证这种可能性，我们研究了热应激对ROS生产，蛋白质含量和AVUCP基因的相关表达的影响，血红素氧酶-1（HO-1）Andnadph氧化酶4（NOX4）在犬骨骨骼肌中的基因初级培养中的细胞。我们还研究了4-羟基 - 速度（Tempol）的还原效果，即在这些参数上是一种强抗氧化剂。

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