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首页> 外文会议>Congress of the European Society for Agricultural and Food Ethics >A tailor-made molecular biological strategy for monitoring the gut microbiota of pigs reflecting animal health
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A tailor-made molecular biological strategy for monitoring the gut microbiota of pigs reflecting animal health

机译：用于监测反映动物健康的猪的肠道微生物的量身定制的分子生物学策略

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The aim of this project was to optimise the methodology for determining the influence of different feeding strategies on the gut microbiota of pigs by using Real-time PCR-based tools. The composition and the physiology of the farm animals gut microbiota are of great interest to science because of their influence on animal health. Animal health again is very important for the economic efficiency and mainly for the safety and quality of animal food. Experience has shown that culture methods often are not capable of satisfactorily describing the real microbial situation in the intestine. Hence, although numerous studies have been introduced during the last years applying this microbial strategy, recent findings have demonstrated that the detection of microbial changes upon feeding can only be accomplished using molecular tools in connection with culture methods. In this study we have compared different methods for monitoring microbial changes in the gastrointestinal tract of pigs as influenced by different feeding strategies. Starting from faecal samples and samples from different gut segments, the total microbial DNA was isolated and different bacterial parameters were quantified by Real-time PCR. The results were compared with those obtained by culture methods and the results of the Fluorescence in-situ Hybridisation (FISH). It was shown that Real-time PCR can be regarded as a fast alternative to conventional techniques, also complementing results obtained from conventional culture methods.
机译：该项目的目的是通过使用基于实时PCR的工具来优化确定不同饲养策略对猪肠道微生物的影响的方法。农场动物肠道微生物的组成和生理学对科学的影响很大，因为它们对动物健康的影响。动物健康再次对经济效率非常重要，主要是为了动物食物的安全和质量。经验表明，培养方法往往能够令人满意地描述肠中真正的微生物情况。因此，虽然在应用这种微生物策略的最后一年期间已经引入了许多研究，但最近的发现表明，饲喂时的微生物变化的检测只能使用与培养方法相关的分子工具来完成。在这项研究中，我们对不同饲养策略的影响进行了比较了不同用于监测猪胃肠道中的微生物变化的方法。从粪便样品和来自不同肠道段的样品开始，分离总微生物DNA，通过实时PCR定量不同的细菌参数。将结果与通过培养方法获得的结果和原位杂交的结果（鱼）。结果表明，实时PCR可以被认为是常规技术的快速替代方案，也可以补充由常规培养方法获得的结果。

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