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首页> 外文会议>International Gluten Workshop >Hypothetical Differential Processing of Low Molecular Weight Glutenin Subunits Met- and Ser-types at Their N-Terminal End
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Hypothetical Differential Processing of Low Molecular Weight Glutenin Subunits Met- and Ser-types at Their N-Terminal End

机译：低分子量谷蛋白亚单元的假设差异加工在其N-末端末端的SER-和SER系列

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摘要

The gluten polymeric fraction is composed by high and low molecular weight glutenin subunits (HMW-GS and LMW-GS, respectively). Typical LMW-GS are classified into two main groups, namely LMW-s and LMW-m types, whose first N-terminal amino acids in themature polypeptides are Ser and Met, respectively, along with a third minor group, LMW-i, storting with He. It has been hypothesized that the presence of the Asn residue instead of a Tiff residue, as it occurs in the supposed LMW-m type sequences, coulddetermine a differential processing at the N-terminal end of LMW-s type sequences, that might generate the cleavage of the peptide MEN by an asparaginyl endoprotease. In order to verify this hypothesis, we have expressed two LMW-GS in Nicotiana benthamiana by using an episomal vector based on Potato Virus X (PVX). The two LMW-GS expressed are represented by two wild types LMW-m and LMW-s genes, along with the mutated forms at position 23. Preliminary results give indications of occurrence of such a differential gene processing at post translational level, at least in the LMW-m type.
机译：面筋聚合物部分通过高和低分子量的蛋白亚基（HMW-GS和LMW-GS，分别地）组成。典型LMW-GS被分为两个主要的组，即LMW-S和LMW-m的类型，它的第一N末端氨基酸中themature多肽是丝氨酸和Met，分别与第三次要组，LMW-I，议会沿用他。已经假设，所述天冬酰胺的存在残基，而不是一个TIFF残余物，因为它在假定的LMW-米型序列发生时，coulddetermine差分在LMW-S型序列的N-末端加工，这可能产生由天冬酰胺酰内切蛋白酶肽MEN的切割。为了验证这一假设，我们通过使用基于马铃薯X病毒（PVX）的附加型载体表达在烟草2个亚基-GS。表示由两个野生型LMW-m和LMW-S基因代表的两个LMW-GS，用突变的形式沿着在位置23的初步结果在翻译后水平得到这样的差异性基因处理的发生的指示，至少在所述LMW-m的类型。

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