• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>American Institute of Chemical Engineers Annual Meeting >CELL CAPTURE BEHAVIOR IN CEE~(TM) MICROFLUIDIC RARE CELL CAPTURE AND ENRICHMENT SYSTEM
【24h】

CELL CAPTURE BEHAVIOR IN CEE~(TM) MICROFLUIDIC RARE CELL CAPTURE AND ENRICHMENT SYSTEM

机译：CEE〜（TM）微流体稀有细胞捕获和富集系统中的细胞捕获行为

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Rare cell extraction and enrichment has long been recognized as a valuable tool for diagnostic medicine. Biocept had identified the emerging technology of microfluidics and bioMEMS to be well suited for diagnostic applications, for which we’ve developed the Cell Enrichment and Extraction (CEE~(TM)) technology platform. CEE~(TM) enriches rare cells from large biological volumes by combining antibody-functionalized surfaces with a microfluidics channel. Cells flowing through the CEE system are captured along a randomized array of functionalized posts, and subsequent processing makes medical diagnosis from rare cells possible. The characteristics of cell capture under different physical conditions were explored experimentally, using JEG-3 choriocarcinoma and K562 erythroleukemia cells as models of specific capture. Capture efficiencies were recorded for channels at various flow rates, antibody concentrations, and angles to the horizontal plane. Cell locations were also noted in terms of capture on the posts or the channel floor, as well as their distance along the channel. We observed higher capture efficiency at greater run angles and flow rates under the conditions investigated. More cells were captured on posts vs. channel floor at higher flow rates.
机译：罕见的细胞提取和富集长期被认为是诊断医学的宝贵工具。生物科e已经确定了微流体和生物核心的新出现技术，非常适合诊断应用，我们开发了细胞富集和提取（CEE〜（TM））技术平台。 CEE〜（TM）通过将抗体官能化的表面与微流体通道组合来富集来自大生物体积的稀有细胞。流过CEE系统的细胞沿着随机化的官能化柱捕获，随后的处理使得来自稀有电池的医学诊断成为可能。通过实验，使用JEG-3 Choriocarcinoma和K562 erythroleukemia细胞作为特定捕获的模型，通过实验进行不同身体状况下进行细胞捕获的特征。以各种流速，抗体浓度和水平平面角度的通道记录捕获效率。在柱子或沟道楼层上的捕获方面还注意到单元位置，以及沿着通道的距离。在调查的条件下，我们观察到更高的捕获效率和流量的流速。在较高流速下捕获更多细胞与沟道底部的柱子。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号