• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Conference on Integration and Commercialization of Micro and Nanosystems >Synthesis and Characterization of Lysine Modified Chitosan Magnetic Microspheres - A Novel Gene Delivery System
【24h】

Synthesis and Characterization of Lysine Modified Chitosan Magnetic Microspheres - A Novel Gene Delivery System

机译：赖氨酸改性壳聚糖磁性微球的合成与表征 - 一种新型基因递送系统

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This paper describes a new formulation of magnetic nanoparticles coated by a novel polymer lysine modified chitosan (CS-lys) as gene carrier. Lysine modified chitosan was synthesized by performing carboxyl of lysine and amido of chitosan in the presence of l-Ethyl-3-(3-dimethyllaminopropyl) carbodiimide hydrochloride (EDO . The absolute chemistry of the Lysine modified chitosan obtained were characterized using IR and H NMR, respectively. The results indicated that many amines of chitosan were modificatied with lysine,and optimized the correlation conditions. The lysine modified chitosan magnetic microspheres were preprated by co-precipitation method. The characterizations of microspheres were measured by TEM, XPS,Dynamic Laser Light Scattering, Vibrating Samples Magnetometer, and X-ray diffraction. The resultes indicated the average particle size was about 100nm and have good superparamagnetic property. The cytotoxicity of CS-lys MNPs was investigated with U293 cells. The results showed that the CS-lys MNPs retained of low toxicity; additionally, combination of DNA and CS-lys MNPs was observed from agarose gel electrophoresis, suggesting that the CS-lys MNPs could be a novel magnetic targeting gene carrier. We also studied the ability of complexes of CS-lys MNPs and DNA crossing BBB in rats by single photon emission computed tomography (SPECT).
机译：本文介绍了由新型聚合物赖氨酸改性的壳聚糖（CS-Lys）作为基因载体涂覆的磁性纳米颗粒的新配方。通过在L-乙基-3-（3-二甲基丙基丙基）碳二亚胺盐酸酯（edo氯酰胺的存在下，通过在氯氨籽和壳聚糖的羧基（eDO）存在下合成赖氨酸改性的壳聚糖。用IR和H NMR表征获得的赖氨酸改性壳聚糖的绝对化学物质分别表明，壳聚糖的许多胺在赖氨酸正相着，并优化了相关条件。通过共沉淀法预先进行赖氨酸改性的壳聚糖磁性微球。通过TEM，XPS，动态激光测量微球的表征。散射，振动样品磁力计和X射线衍射。结果表明平均粒度为约100nm并具有良好的超顺磁性。用U293细胞研究CS-Lys MNP的细胞毒性。结果表明CS-Lys MNPS保留低毒性;另外，从琼脂糖凝胶电泳中观察到DNA和CS-Lys MNP的组合是，表明CS-Lys MNP可以是新型磁靶向基因载体。我们还通过单光子发射计算机断层扫描（SPECT）研究了CS-Lys MNP和DNA交叉BBB的复合物的能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号