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Engineering of Efficient Biocatalysts Using Nanostructured Mesoporous Silicate Carriers

机译:利用纳米结构型硅酸盐载体的高效生物催化剂的工程

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Comparative studies of the biocatalysts with invertase immobilized on three nanostructured silicate carriers: mesoporous cellular foams and two types of SBA-15 materials using three highly recommended protein bonding methods are reported. Invertase was attached to the surface by: physical adsorption followed by its crosslinking, adsorption on aminated MS followed by its crosslinking, and covalent bonding with aminated MS via GLA spacer. Experiments clearly indicate that activity of the aminated MCF-based biocatalysts is significantly higher than of both SBA-15-based counterparts The microenvironment of proteins and porous texture of supports appear the most important factors for enzyme activity expression, and in this respect the unique properties of aminated MCFs can hardly be matched
机译:据报道,倒反转酶的生物催化剂的比较研究报道了三种纳米结构硅酸盐载体的逆变酶:介绍了使用三种高度推荐的蛋白质键合方法的中孔细胞泡沫和两种类型的SBA-15材料。通过:物理吸附,其交联,对其交联,对胺型MS的吸附后,并通过GLA间隔物与胺化MS共价键合。实验清楚地表明,胺化的基于MCF的生物催化剂的活性显着高于SBA-15的对应物,蛋白质的微环境和多孔纹理的助剂似乎是酶活性表达的最重要因素,并且在这方面是独特的性质常规的MCF可以匹配

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