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首页> 外文会议>IASTED International Conference on Nanotechnology and Applications >NANORELIEF COATINGS WITH INCREASED SURFACE AREA OF FUNCTIONAL GROUPS FOR DNA IMMOBILIZATION
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NANORELIEF COATINGS WITH INCREASED SURFACE AREA OF FUNCTIONAL GROUPS FOR DNA IMMOBILIZATION

机译：具有增加的官能团表面积的纳米光涂层，用于DNA固定化

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摘要

The coatings described in this work are based on filmforming organic polymers with grafted oligosiloxane chains. The composition was synthesized by acidcatalyzed hydrolysis and condensation of alkoxysilanes in the presence of polymers bearing active groups, capable of reaction with alkoxysilanes or products of their hydrolysis: hydroxy, acetal, epoxy, vinyl ether. Microphase layering between 3D silica nanoparticles and polymeric chains results in nanorelief "hilly" surface of the film. Oligosiloxane particles obtained from alkoxysilanes are chemicaly active which allows to introduce various functional groups during synthesis of the coatings. The resulting surfaces show high density of the desired groups: up to 900 nm~(-2) in the case of aminogroups and up to 300 nm~(-2) for epoxy, carboxy and hydrazide moieties. Covalent immobilization of oligonucleotide to these coatings opens way to new systems of genetic analysis and purification of nucleic acids. Surface density of immobilized oligonucleotide active in hybridization was up to 10 pmol/mm~2. We also hope new coatings and freestanding films could be useful in design of immune and chemical sensors.
机译：本作作品中描述的涂层基于具有接枝的寡硅氧烷链的成膜有机聚合物。该组合物通过酸催化水解和烷氧基硅烷在含有活性基团的聚合物存在下合成，能够与其水解的烷氧基硅烷或产物反应：羟基，缩醛，环氧树脂，乙烯基醚。 3D二氧化硅纳米粒子和聚合物链之间的微相层导致薄膜的纳米“丘陵”表面。从烷氧基硅烷获得的寡聚硅氧烷颗粒是化学活性的，其允许在合成涂层期间引入各种官能团。所得表面显示出高密度的所需基团：在氨基小组的情况下，高达900nm〜（-2），对于环氧树脂，羧基和酰肼部分最高可达300nm〜（-2）。将寡核苷酸的共价固定到这些涂层上，为核酸的遗传分析和纯化的新系统打开了新的遗传分析系统。固定化寡核苷酸的表面密度在杂交中最高可达10pmol / mm〜2。我们还希望新的涂料和独立薄膜可用于设计免疫和化学传感器。

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