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Establishment and characterization of a dairy cows pituitary cells model for lactation hormones study

机译：乳制奶牛垂体细胞哺乳荷尔蒙研究的建立与表征

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Introduction Growth hormone (GH) and prolactin(PRL) which secreted by the anterior pituitary gland play a key role in the regulation of milk protein and fat synthesis(Akers 2006).Because of the irreplaceable regulatory role in lactation,researches on endocrinology of lactation were always focuses on the factors and mechanism affecting GH and PRL synthesis and release.Cell culture is a convenient tool forunderstandingthe mechanisms of hormone synthesis and secretion.Howeve,existing in vitro approaches are noteffective for the investigation of GH and PRL synthesis and secretion in dairy cow anterior pituitary gland.Thus,a primary objective of this investigation was to isolate and cultureanterior pituitary cells in vitro so that their potential as a model to study the cellular and molecule mechanism for GH and PRL synthesis and secretion.
机译：引入前垂体腺体分泌的生长激素（GH）和催乳素（PRL）在牛奶蛋白和脂肪合成的调节中发挥关键作用（AKERS 2006）。因为对哺乳期的不可替代的监管作用，对哺乳期内分泌的研究始终专注于影响GH和PRL合成和释放的因素和机制.Cell培养是一种方便的工具，是一种方便的工具，其缺乏激素合成和分泌机制.Howeve，现有的体外方法对乳制品的研究进行调查，对GH和PRL合成和分泌说明是说明的母牛前垂体腺体。本研究的主要目的是在体外分离和养殖垂体细胞，使其作为研究GH和PRL合成和分泌的细胞和分子机制的模型的潜力。

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