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Synergistic antimicrobial activity among hydroalcoholic extract of leaves of trees in the Brazilian territory common

机译:巴西领土叶片叶片水醇提取物中的协同抗菌活性

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Many plants are used in Brazil to treat common infections without any scientific evidence of efficacy. This study aimed to evaluate the antimicrobial activity of hydroalcoholic extract from leaves of Eugenia uniflora, Punica granatum and association between the extracts against Staphylococcus aureus. The antibacterial activities of extracts were determined by macrodilution techniques in BHI broth. The minimum inhibitory concentration (MIC90) was determined by measuring the optical density in the spectrophotometer and was defined as the lowest concentration of crude extract that produced a 90% reduction in visible growth compared with non inoculated broth. All concentrations of extracts and association of extracts from the leaves of the plants tested showed some inhibition of bacterial growth. However, only the combination of the extracts was obtained MIC90 (0.5 mg/mL). Inhibitory activity on bacterial growth of the association of the extract from leaves of P. granatum with E. uniflora was higher when compared with the inhibition of bacterial growth promoted by extracts alone, indicating a bacteriostatic synergistic effect between these two extracts.
机译:在巴西使用许多植物治疗常见感染,没有任何科学的疗效。该研究旨在评估桉树植物叶片的水醇提取物的抗微生物活性和蛋白质蛋白菌葡萄球菌的萃取物之间的关系。提取物的抗菌活性由BHI肉汤中的大分子技术确定。通过测量分光光度计中的光学密度来确定最小抑制浓度(MIC90),并且与非接种肉汤相比,被定义为产生的可见生长90%的粗提取物的最低浓度。所有浓度的提取物和从植物叶片的提取物相关的浓度和相关性的测试表现出一些抑制细菌生长。然而,仅获得萃取物的组合MIC90(0.5mg / ml)。当与单独提取物促进的细菌生长相比,抑制来自P.叶片的叶片的细菌生长的抑制活性。与单独提取物促进的细菌生长相比,表明这两种提取物之间的抑菌协同效应。

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