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Genetic variability analysis of entomopathogenic fungi isolated from citrus-growing areas of Mexico

机译：墨西哥柑橘生长区昆虫致病真菌的遗传变异分析

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The objective of the study was to isolate and characterize genetically native fungi that remain in the soil of the diverse citrus regions of Mexico. We collected 142 soil samples from different Mexican states: Campeche (13), Michoacán (15), Nuevo León (25), San Luis Potosí (25), Sinaloa (17), Sonora (17), Tabasco (5), Tamaulipas (20), and Yucatán (5). Larvae of Galleria mellonella were used as bait to detect, trap and multiply entomopathogenic fungi in vivo. 23% of the soil samples processed were positive for the presence of entomopathogenic fungi according to the macroscopic and microscopic characteristics: Beauveria was detected in 12% (17 isolates), Metarhizium in 1% (2 isolates) and Isaria in 10% (14 isolates). We analyzed the genetic variability of the fungi by using the sequences of internal transcribed ribosomal genes (ITS-5.8S-ITS2) and subsequently performed the digestion with the enzyme Hae III. The results of the PCR generated fragments of approximately 600 bp for Beauveria spp., and Metarhizium spp. In the case of Isaria spp., it amplified a fragment above of 600 bp. Both reference strains and field isolates produced similar restriction patterns. Genera of native entomopathogenic fungi were found on the diverse citrus areas of Mexico; this opens the field for the biological control of pests affecting citrus in each region.
机译：该研究的目的是分离和表征遗传原生真菌，留在墨西哥各种柑橘地区的土壤中。我们收集了来自墨西哥不同墨西哥国家的土壤样本：坎佩切（13），Michoacán（15），NuevoLeón（25），Sinaloa（17），Sonora（17），Tabasco（5），Tamaulipas（ 20）和Yucatán（5）。 Galleria Mellonella的幼虫用作诱饵，以检测，捕获和乘以体内昆虫致死真菌。根据宏观和微观特征，23％的土壤样品对昆虫致病真菌的存在是阳性的：在12％（17个分离物）中，1％（2分离株）和Isaria以10％（14个分离物）检测到Beauveria ）。我们通过使用内部转录的核糖基因基因（其-5.8S-ITS2）的序列分析了真菌的遗传可变性，随后用酶Hae III进行消化。 PCR产生的嗜孢子菌的PCR产生约600bp的碎片。和Metarhizium spp。在Isaria SPP的情况下，它扩增了600 bp以上的片段。参考菌株和场隔离产生类似的限制模式。在墨西哥的多样化柑橘地区发现了原生昆虫疗法真菌的属;这为影响每个地区影响柑橘的害虫的生物控制领域。

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