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The decolorization of azo dyes by Trametes versicolor under the influence of different glucose concentrations

机译:在不同葡萄糖浓度的影响下,Trametes Versicolor的脱色亚氮染料

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Various azo-dyes can be found in environment as a result of textile industry activities and most of them are harmful for human and animal health. The decolorization and removal of most of these compounds usually requires complex physico-chemical treatment of the wastewaters and is not quite effective. The following biological treatment where the main components are microorganisms becomes still more important. The white rot fungi are distinguished among most of the microbial strains discussed in the literature for its ability to decolorize various industrially applied dyesA white rot fungi Trametes versicolor strain capable of decolorizing various textile dyes was investigated in this study. The strain identification was performed on the basis of 18S rDNA analysis (Acc No GQ472774). The decolorization of two widely used azo dyes (Orange II, Congo Red) was followed in conditions of mineral medium cultivation with different concentrations of dye (50mg/l and 125mg/l) and glucose (1, 2 and 3%) included in a medium. The strain ability to decolorize listed azo - dyes was established by UV-Vis spectroscopy of samples taken before and at every 24 hours during dye decolorization in the culture medium. The processes of decolorization were performed under aerobic condition at pH 6.5 and 28 °C.The data obtained gave proof of the fact that higher glucose concentration actually lowered the specific decolorization rate. Nevertheless, the biggest percent of decolorization observed in a medium containing 3% glucose is due to the longer time of growth and development of microbial culture and respectively longer laccase production. The observed laccase (EC 1.10.3.2) activity dynamics indicated the involvement of this enzyme in the decolorization processes.The best results were obtained in a cultivation medium complemented with 3% glucose in which the strain Trametes versicolor 1 decolorized 125 mg/l of each investigated dye as follow: 90% of Orange II within 336 h and 90 % of Congo Red within 360 h.
机译:由于纺织业活动,各种偶氮染料可以在环境中找到,并且它们中的大多数对人类和动物健康有害。大多数这些化合物的脱色和去除通常需要对废水的复杂物理化学处理,并不完全有效。以下生物处理主要成分是微生物的变得更为重要。在该研究中,在文献中讨论的大多数微生物菌株中的大多数微生物菌株中的大多数微生物菌株的特征在于,在本研究中研究了能够脱色各种纺织染料的抗真菌菌株的葡萄球菌菌株。基于18S RDNA分析进行应变鉴定(ACC NO GQ472774)进行。在具有不同浓度的染料(50mg / L和125mg / L)和葡萄糖(1,2和3%)的矿物质培养条件下,遵循两种广泛使用的偶氮染料(橙色II,刚果红色)的脱色。中等的。通过在培养基中的染料脱色之前和每24小时以前和在每24小时之前和在培养基中的每24小时以前和每24小时进行脱滤的脱氮染料的应变能力。在pH6.5和28℃下在有氧条件下进行脱色的方法。获得的数据证明了更高的葡萄糖浓度实际降低了特异性脱色率。然而,在含有3%葡萄糖的培养基中观察到的最大偏差百分比是由于微生物培养的较长时间和发育的时间较长,并且分别更长的漆酶生产。观察到的漆酶(EC 1.10.3.2)活性动态表明该酶在脱色过程中的介入。在培养介质中获得的最佳结果是互补的3%葡萄糖,其中菌株Trametes versicolor 1脱色125mg / L.所研究的染料如下:90%的橙色II在336小时内,90%的刚果红色在360小时内。

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