Evaluation of flow cytometry to assess Erwinia amylovora viability under different stress conditions

机译：流式细胞术评估不同应力条件下的Erwinia Amylovora活力的评估

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Erwinia amylovora is the causal agent of fire blight, one of the most devasting and difficult to control diseases of pear and apple trees. There is scarce information about the survival mechanisms of the pathogen outside the host, but it has been demonstrated that it enters into the viable but non-culturable (VBNC) state under different stresses. In that physiological state, bacterial cells are viable but unable to grow on the general media where they usually do. Therefore, culture-independent methods, such as epifluorescence microscopy (EFM) combined with a viability staining, are necessary to monitor bacterial survival. Nevertheless, microscopic counts have some limitations which can be solved with other techniques like flow citometry (FCM). We have evaluated FCM, after Live/Dead staining, to assess E. amylovora viability under diverse stresses, using EFM in parallel. Results have shown that FCM allows a clearer and faster discrimination of E. amylovora viable and non-viable cells than EFM, so it can be applied to study the physiological state of the pathogen under diverse adverse conditions.

机译：Erwinia Amylovora是火灾枯萎的因果因子，是最繁重，难以控制梨和苹果树的疾病之一。有关宿主外病原体存活机制的稀缺信息，但已经证明它在不同应力下进入可行但非培养的（VBNC）状态。在这种生理状态下，细菌细胞是可行的，但无法在他们通常做的总媒体上生长。因此，必须与活性染色相结合的培养无关的方法，例如离荧光显微镜（EFM），以监测细菌存活。然而，微观计数具有一些限制，其可以用其他技术如流动性（FCM）求解。我们在实时/死染色后评估了FCM，以评估在不同的应力下的氨基罗沃生活力，并行使用EFM。结果表明，FCM允许更清晰，更快地辨别E.淀粉醇可行和非活性细胞的辨别比EFM，因此可以应用于研究病原体的生理状态在不同的不利条件下。

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《International Conference on Environmental, Industrial and Applied Microbiology》|2009年||共4页
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R.D. Santander; J.F. Catalá-Senent; M. Ordax; A. Flores; E. Marco-Noales; E.G. Biosca;
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中图分类 Q939.9-532;
关键词
phytopathogenic bacterium; fire blight; cytometry; epifluorescence microscopy; chlorine; copper; acetic acid; hydrogen peroxide; VBNC;

机译：植物疗法细菌;火焰枯萎;细胞计数术;离荧光显微镜;氯;铜;醋酸;氢过氧化氢;VBNC;

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Evaluation of flow cytometry to assess Erwinia amylovora viability under different stress conditions

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