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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Reactive Violet 12 dye decolorization by mycelial culture of Trametes versicolor
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Reactive Violet 12 dye decolorization by mycelial culture of Trametes versicolor

机译：反应性紫罗兰12染料脱色，Trametes versicolor

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The industry is a major source of pollution for water ecosystems. Industrial production of textile, cellulose and various chemicals is connected with synthetic dyes usage. The discharged effluents could have a hazardous influence on the environment. The biological treatment for synthetic dyes removal is a very perspective, environmentally protective and low cost approach for solution of such problems. One of the recently wide used in dyeing of cellulose fabrics and textile industry dyes is an antraquinon dichloro triazine Based Dye, known as Reactive Violet 12. Its popularity is due to the effective cold dying properties. There is not any data in the scientific literature about the process of biodecolorization of wastewater obtained after treating with this dye. The process of Reactive Violet 12 decolorization by Trametes versicolor strain 1 was investigated in this study. The experiments were carried out with different concentrations of dye (50mg/l and 125mg/l) and glucose (1,2 and 3%) in a medium. The highest values of the specific decolorization rates (Q) of the processes carried out with different glucose concentrations were obtained in the presence of 1% glucose. The enzyme activity of laccase (EC 1.10.3.2) was measured during the process of decolorization. A direct correlation between the observed enzyme activity and the effectiveness of investigated processes was proved. In spite of the fastest speed of decolorization registered in the medium complemented with 1% glucose the total decolorization was improved by increasing the initial glucose concentration in the medium. The higher concentrations of glucose maintained more percentage of decolorization due to the better growth of Trametes versicolor. Nevertheless it was established that maximal laccase activity was comparably equal in all described experiments (120 U/ml - 145 U/ml). It was established that the best conditions for laccase production are in a medium containing 3% glucose. Correspondingly, the decolorization of 125mg/l Reactive Violet 12 dye in these conditions was 100 % completed in 360 hours.
机译：该行业是水生态系统污染的主要来源。工业生产纺织，纤维素和各种化学品与合成染料使用相连。排出的流出物可能对环境产生危险影响。除去合成染料的生物处理是一种非常透视，环保和低成本的方法，用于解决这些问题。最近用于纤维素织物和纺织工业染料中的染色的宽度是一种基于Antraquinon二氯三嗪的染料，称为反应性紫罗兰12.它的普及是由于有效的冷染性能。关于用这种染料处理后获得的废水的生物焦化过程中没有任何数据。在本研究中研究了Trametes Versicolor菌株1的反应性紫色12的过程。在培养基中以不同浓度的染料（50mg / L和125mg / L）进行实验，并在培养基中进行葡萄糖（1,2和3％）。在1％葡萄糖存在下获得具有不同葡萄糖浓度的方法的特异性脱色速率（Q）的最高值。在脱色过程中测量漆酶（EC 1.10.3.2）的酶活性。证明了观察到的酶活性与调查过程的有效性之间的直接相关性。尽管在辅助1％葡萄糖的介质中登记的脱色速度最快，通过增加培养基中的初始葡萄糖浓度来改善总脱光。由于Trametes Versicolor的较好增长，较高浓度的葡萄糖浓度保持多百分比的脱色。然而，确定最大漆酶活性在所有描述的实验中相当相等（120U / mL-145 U / mL）。建立了漆酶生产的最佳条件是含有3％葡萄糖的培养基。相应地，在这些条件下125mg / L反应性紫12染料的脱色为100％在360小时内完成。

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