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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Isolation and characterization of cellulase producing bacteria from pruning tree compost and soil
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Isolation and characterization of cellulase producing bacteria from pruning tree compost and soil

机译：纤维素酶产生细菌的分离与表征修剪树堆肥和土壤

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Twenty-seven strains of moderately thermophilic bacteria, which degraded microcrystalline cellulose or carboxymethylcellulose (CMC), were isolated from pruning tree compost and soil. The 16S rDNA sequence analysis indicated that these isolates were classified into four groups in species level. The two groups of the four showed less than 95% similarity with any published 16S rDNA sequences and were related to the sequences of Paenibacillus spp. This result indicated that isolates of these two groups are novel species of the genus Paenibacillus or possibly novel genus. Strains SCA3a and strain TC22-2b were selected as representatives of the strains of the two groups and were characterized physiologically and chemotaxonomically. The optimum temperature and pH for growth of both SCA3a and TC22-2b were around 55°C and 7-8, respectively. This optimum temperature was at least 20°C higher than that of closly related published Paenibacillus species. Also the major fatty acid compositions of strain SCA3a and TC22-2b were not similar to the related species.
机译：中度嗜热菌，其降解微晶纤维素或羧甲基纤维素（CMC），购自修剪树堆肥和土壤中分离的二十七个菌株。该16S rDNA序列分析表明，这些菌株被分为四组在物种水平。四个两组显示出与任何公布的16S rDNA序列小于95％的相似性和均与类芽孢杆菌属的序列。这一结果表明，这两个基团的分离物是类芽孢杆菌属或可能新的属的新种。菌株SCA3a和应变TC22-2b被选定为两组的菌株的代表并生理学和chemotaxonomically表征。的最适温度和pH值两者SCA3a的生长和TC22-2b为分别围绕55℃和7-8。该最佳温度为至少20℃比closly相关出版类芽孢杆菌物种的更高。还应变SCA3a和TC22-2b的主要脂肪酸组合物是不相似的相关物种。

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