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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Identification of molds associated with green table olives
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Identification of molds associated with green table olives

机译：鉴定与绿色表橄榄相关的模具

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Mold growth on table olives may be implicated in different problems of spoilage, one of which is related to their ability to produce mycotoxins. In this study twelve mold strains were isolated during the processing of green table olives. These molds were identified by studying their macroscopic and microscopic characteristics, using restriction analysis of ITS1-5.8s rDNA-ITS2, and according to their secondary profiles of metabolites analyzed by micellar electrokinetic capillary chromatography (MECC). This was confirmed with a 5.8-ITS region sequence analysis. The results showed that five of the isolates belong to Penicillium, three to Aspergillus, two to Fusarium, one to Beauveria, and two were identified as the yeast-like fungus, Galactomyces. The ability of the isolates to synthesize mycotoxins on malt extract agar was investigated by MECC. Four strains produced a total of three mycotoxins: 3 strains, identified as Penicillium expansum, produced roquefortine, and 1, identified as Aspergillus flavus, produced two mycotoxins, cyclopiazonic acid and aflatoxin B_1.
机译：表橄榄中的模具生长可能涉及腐败的不同问题，其中一个与其生产霉菌毒素的能力有关。在这项研究中，在绿色表橄榄处理过程中分离了12种模具菌株。通过研究其宏观和微观特性，使用其1-5.8s rdna-ITS2的限制分析，并根据胶束电动毛细管色谱（MECC）分析的代谢物的二次谱来鉴定这些模具。通过5.8-其区域序列分析证实了这一点。结果表明，五个分离物属于青霉素，三个到曲霉，两个到镰刀菌，一个到嗜生物，两者和两种被鉴定为酵母样真菌，加乳胺。用MECC研究了分离物在麦芽提取物琼脂上合成霉菌毒素的能力。四种菌株共产生三种霉菌毒素：3个菌株，被鉴定为青霉植物，产生的罗基摩特和1，鉴定为曲霉素，产生两种霉菌毒素，环偶酸和黄曲霉毒素B_1。

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