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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Ultrastructural behavior of Rhodotorula mucilaginosa induced by the growth in presence of pyrene
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Ultrastructural behavior of Rhodotorula mucilaginosa induced by the growth in presence of pyrene

机译：乳烯生长诱导rhodotorula粘液蛋白酶的超微结构行为

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摘要

The present study was carried out in order to evaluate the resistance / tolerance of an isolate Rhodotorula mucilaginosa, single-celled fungal, related to the ultrastructural behavior by the growth in pyrene, a polycyclic aromatic hydrocarbon (PAH). The organism was grown in Yeast Mold Broth (YMB) in the absence and presence of pyrene at concentrations of 0.25mg/mL, 0.5mg/mL and 1mg/mL, under orbital agitation of 150 rpm and 28°C. Samples were collected at 24h of cultivation. The detection of the ultrastructural alterations was performed by the use cytochemical techniques for transmission electron microscopy. The ultrastructural analysis of R. mucilaginosa using the routine technique revealed alterations in the fine structure of cells, related to the presence of vacuoles, cell wall electron density, number of eletrondense bodies and mitochondria. Cultures treated with pyrene exhibited differences on the staining pattern when compared to control cells and pyrene concentrations. The cytochrome oxidase and catalase cytochemistry showed variations in the intensity and distribution of reaction products in the cell wall, cytoplasmic membrane, cytoplasm and mitochondria related to the pyrene concentration used during cellular growth. In the experimental conditions used in this work the presence the pyrene induced different effects on R. mucilaginosa ultrastructure, which are presented for the first time in the literature.
机译：进行了本研究，以评估孤立核霉菌的抗性/耐受性，单细胞真菌，与芘的生长，多环芳烃（PAH）的生长有关。在玉米酮的浓度下在0.25mg / ml，0.5mg / ml和1mg / ml的浓度下，在玉米液的情况下在酵母液（YMB）中生长在酵母中，在眶下搅拌下为150rpm和28℃。在培养的24小时内收集样品。通过用于透射电子显微镜的使用细胞化学技术进行超微结构改变的检测。使用常规技术R. mucilaginosa的超微结构分析揭示了细胞精细结构的改变，与存在真空，细胞壁电子密度，Eletrondense体数和线粒体的存在。与对照细胞和芘浓度相比，用芘处理的培养物表现出染色模式的差异。细胞色素氧化酶和过氧化氢酶细胞化学显示了与在细胞生长期间使用的芘浓度相关的细胞壁，细胞质膜，细胞质和线粒体中的反应产物的强度和分布的变化。在该工作中使用的实验条件下，芘的存在诱导对粘酰胺超微结构的不同作用，这在文献中首次呈现。

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