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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Effect of a respiratory inhibitor on the bioconversion of a xenobiotic by activated sludge
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Effect of a respiratory inhibitor on the bioconversion of a xenobiotic by activated sludge

机译：呼吸抑制剂对活性污泥生物转化症的影响

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摘要

The bioconversion of xenobiotics often involves oxidation-reduction reactions and one representative example is the reductive decolorization of azo dyes. The bioreduction of an azo dye as model xenobiotic by a mixed microbial culture (activated sludge) was thus chosen as a model system in the present study. The aim was to investigate the effect of a respiratory inhibitor, namely sodium azide (a cytochrome oxidase inhibitor) on this bioconversion, when carried out by two different activated sludge mixed culture inocula. Different biomass conditioning pretreatments were also studied, i.e., using a mixed culture freshly harvested from the growth bioreactor or after aerobic incubation in the absence of carbon source (starved). The azide inhibition effect was observed to lessen with the increase in starvation time for both mixed cultures. This result points to the existence of an alternative dye bioconversion mechanism, triggered in the starved biomass, in which the involved pathways are not inhibited by azide. For one of the inocula, this alternative mechanism was found to be dependent on the availability of glucose in the bioconversion medium.
机译：异卵生物的生物转化常涉及氧化还原反应，并且一个代表性实例是偶氮染料的还原脱色。因此，通过混合微生物培养（活性污泥）作为模型异丙酸的偶氮染料的生物测量作为本研究中的模型系统。目的是探讨呼吸抑制剂，即叠氮化钠（细胞色素氧化酶抑制剂）对这种生物转化的影响，当通过两种不同的活性污泥混合培养培养接种时进行。还研究了不同的生物质调理预处理，即，使用从生长生物反应器新鲜收获的混合培养物或在不存在碳源（饥饿）的情况下进行的混合培养物。观察到叠氮化物抑制效果，以减少混合培养物的饥饿时间的增加。该结果指向在饥饿生物质中触发的替代染料生物转化机制的存在，其中所涉及的途径不受叠氮。对于其中一个接种症，发现该替代机制依赖于生物转化介质中葡萄糖的可用性。

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