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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Survival and persistence of Bacteroides species as faecal indicators and the recovery of 16S rRNA markers under controlled conditions
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Survival and persistence of Bacteroides species as faecal indicators and the recovery of 16S rRNA markers under controlled conditions

机译：将菌体种类的存活率和持续存在于受控条件下的16S rRNA标志物的归还和恢复

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摘要

The persistence of faecal Bacteroides species after release into differing environmental waters and sediments is not well understood. This 18 day study aimed to determine the survival (using culture-based method) and PCR-based marker recovery of human- and dog-sourced Bacteroides from river and seawater laboratory microcosms containing muddy sediment. The survival of Bacteroides in the water declined very sharply from 1.2×10~4 cfu/100 ml to near zero between day 6 and day 14; likewise for those in the sediment a rapid decline was observed from 8.0× 10~3 cfu/gram to near zero between day 8 and day 16. Generic, human and dog Bacteroides PCR primers were used to evaluate the persistence of faecal Bacteroides markers in these controlled conditions and the rates of decline were similar to culture-based recovery. The similarity in persistence of these Bacteroides 16S rRNA genetic markers in environmental water and sediment suggests that viable but non-culturable (VBNC) Bacteroides do not persist in the natural environment for long and therefore that 16S rRNA markers can thus be of value as faecal indicators of bathing water pollution and in source tracking.
机译：粪便诱导物种释放到不同的环境水域和沉积物后的持续性尚不清楚。这项18天的研究旨在确定来自河流和含有泥泞沉积物的海水实验室微观的人和狗质生物诱导的基于PCR的存活率和基于培养基方法）和PCR的标记。在第6天和第14天之间，水中拟枝杆菌的存活率从1.2×10〜4 CFU / 100ml到零接近零;同样，对于那些在第8天和第16天之间的8.0×10〜3 CFU /克接近零的沉淀物中的那些，使用了快速下降。通用，人和犬杆菌PCR引物用于评估这些粪便菌体标志物的持续性受控条件和下降率类似于基于培养的恢复。在环境水和沉积物这些杆菌的16S rRNA遗传标记的持久性的相似性表明，活的非可培养（VBNC）拟杆菌不会在自然环境中持续很长，并且因此其16S rRNA的标记物可因此是有价值的作为粪便指标沐浴水污染与源跟踪。

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