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Activities Change of Amylase in Chinquapin during Fruit Development

机译:果实开发期间Chinquapin淀粉酶的活动变化

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Chinquapin (Castanea henryi Rehd. & Wils.) is an important fruit species in northern Fujian province. The changes of α-amylase and β-amylase activities were studied. The chinquapin cotyledon and pericarp in different development stages were used. Α and β-amylase were determined by spectrophotometer, and the OD (optical density) value were defined as the activity of amylase. The results showed that the α-amylase activity of chinquapin cotyledon increased gradually during fruit developing, whereas the α-amylase activity of chinquapin pericarp appeared the shape of single peak type. The change degree in different breeds was significant, which was related to the autumn difference in 8 breeds. The difference of a-amylase activities between pericarp and cotyledon was significant in same breed. The α-amylase activity in pericarp was higher than that in cotyledon during fruit development prophase, but the relationship between pericarp and cotyledon was reverse during fruit development anaphase. The results suggested that the fruit α-amylase focus on pericarp mainly in development prophase, and it focus on cotyledon in development anaphase mainly. We conferred that αamylase played an important role in regulating and balancing the form of carbohydrate in fruit. The results showed that the β-amylase activity of chinquapin cotyledon appeared the shape of single peak type. The pericarp β-amylase activity in fruit development prophase was higher than that in anaphase. The pericarp βamylase activity was always higher than that in cotyledon during fruit development. The β-amylase activities were always higher than α-amylase in Pericarp and cotyledon, showing that amylum degradation relied on β-amylase mainly.
机译:Chinquapin(Castanea Henryi Rehd。&Wils。)是福建省北部的重要水果种类。研究了α-淀粉酶和β-淀粉酶活的变化。使用不同发育阶段的Chinquapin cotyledon和Pericarp。通过分光光度计测定α和β-淀粉酶,并将OD(光密度)值定义为淀粉酶的活性。结果表明,Chinquapin子叶的α-淀粉酶活性在果实发育过程中逐渐增加,而Chinquapin Pericarp的α-淀粉酶活性出现了单峰型的形状。不同品种的变化程度是显着的,这与8种品种的秋季差异有关。果皮和子叶之间的淀粉酶活性的差异在同一品种中显着。果皮中的α-淀粉酶活性在水果发育前果发育期间高于子叶中的α-淀粉酶活性,但在果实发育后,果皮和子叶之间的关系逆转。结果表明,果实α-淀粉酶聚焦的主要在发育预言中,它主要关注在发育中的子叶中。我们赋予αamylase在调节和平衡水果中的碳水化合物形式方面发挥着重要作用。结果表明,Chinquapin cotyledon的β-淀粉酶活性出现了单峰型的形状。果实发育预先生果发育前蛋白β-淀粉酶活性高于中源性。在果实发育过程中,Pericarpβamyl酶活性总是高于子叶中的。 β-淀粉酶活性始终高于PericaRP和子叶中的α-淀粉酶,显示出主要依赖于β-淀粉酶的淀粉降解。

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