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ISSR analysis of genetic diversity of rice germplasm with long panicles and big grains

机译：长圆锥和大谷物稻种质遗传多样性的ISSR分析

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The genetic diversity of 56 specific rice germplasm accessions with long panicles (30.73–46.3 cm) and big grains (30.7–70.63 g) was analyzed using inter simple sequence repeats (ISSR). This rice was derived from the IRRI new plant type strains and then developed by crossing and radiation. A total of 80 pairs of ISSR primers were applied to screen for polymorphism, with nine of them being tested to produce polymorphic bands. The nine pairs of primers generated a total of 70 bands, of which 47 were polymorphic. Percentage polymorphic bands (PPB)was 66.18%; genetic similarity coefficient (GS) varied from 0.368 to 0.962,with an average of 0.665; genetic diversity index (H) was 0.233; the number of alleles (Na) was 1.662; and the effective number of alleles (Ne) amounted to 1.414. Clustering analysis showed 56 accessions that could be classified into four groups with GS of 0.64: the first cluster consisted of nine accessions;the second and third clusters contained six accessions, respectively; and the fourth group had 35 accessions. The results provided the scientific basis for further studies on their molecular mechanism and use in breeding.
机译：使用单一简单的序列重复（ISSR）分析56种特异性水稻种质过程的遗传多样性（30.73-46.3cm）和大谷物（30.7-70.63g）分析。这种稻米来自于IRRI新植物型菌株，然后通过交叉和辐射开发。总共80对ISSR引物施加到多态性的筛选中，其中九是测试以产生多态带。九对引物产生了总共70条带，其中47个是多态的。百分比多态性带（PPB）为66.18％;遗传相似系数（GS）从0.368变化到0.962，平均为0.665;遗传多样性指数（h）为0.233;等位基因数量（NA）为1.662;并且有效数量的等位基因（NE）达1.414。聚类分析显示56种附加，可以分为4个组，GS为0.64：第一个群集团由九个群组组成;第二和第三集群分别包含六种载体;第四组有35名豁免。结果为进一步研究其分子机制和繁殖使用提供了科学依据。

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