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首页> 外文会议>International Conference on Environmental Science and Information Application Technology >Loss of Genetic Variation in Hatchery Produced Haliotis Asinina Using AFLP and Allozyme Markers
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Loss of Genetic Variation in Hatchery Produced Haliotis Asinina Using AFLP and Allozyme Markers

机译：使用AFLP和丙醇酶标记产生孵化场遗传变异的遗传变异

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The genetic diversity and differentiation between wild and hatchery Haliotis asinina populations were tested by allozyme and AFLP techniques. The results from allozyme technique showed that there was a decline in mean number of alleles per locus, percentage of polymorphic loci and observed heterozygosity and a change in allele frequency in three loci, SDH-1, AAT-1 and EST-1. Allelic richness, percentage of polymorphic loci and observed heterozygosities ranged from 1.4 (0.2) to 1.2 (0.1), from 30 to 20, from 0.150 (0.104) to 0.140 (0.103) within both wild and hatchery H.asinina populations, respectively. Compared to wild population, the hatchery population from AFLP technique also showed that there was a reduction in effective number of allele, gene diversity, shannon's information index and percentage of polymorphic loci, they ranged from 1.5215 to 1.4675, from 0.2932 to 0.2609, from 0.4252 to 0.3778 and from 69.93 to 62.09, respectively. But low level of differentiation was found between wild and hatchery H.asinina populations. It is inferred that it is small effective population size inbreeding that results in loss of genetic variation in the hatchery H.asinina seed.
机译：通过丙醇酶和AFLP技术测试野生和孵化场卤虫菌菌的遗传多样性和分化。来自酶部技术的结果表明，每个基因座的平均等位基因数量下降，多态性基因座的百分比和观察到的杂合性以及等位基因频率的变化，在三个基因座中，SDH-1，AAT-1和EST-1。等位基因丰富，多晶型基因座和观察到的杂合子的百分比范围为1.4（0.2）至1.2（0.1），分别为野生和孵化器H.asinina群中的0.150（0.104）至0.140（0.103）。与野生种群相比，来自AFLP技术的孵化场群体还表明，有效数量的等位基因，基因多样性，香农信息指数和多态性基因座的百分比，它们从1.5215到1.4675，从0.2932到0.2609，从0.4252分别为0.3778和69.93至62.09。但在野生和孵化场H.asinina群体之间发现了低级别的分化。推断它是小的有效种群尺寸近亲繁殖，导致孵化场H.asinina种子的遗传变异丧失。

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