Yeast two-hybrid system based screening of caspase-8 interacting proteins, and co-immunoprecipitation validation

机译：基于酵母双杂化系统的Caspase-8相互作用蛋白筛选，以及共免疫沉思验证

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Caspase-8, also called FLICE, is involved in the apoptosis process of eukaryotic cells. Recent research has showed that caspase-8 is implicated in both cell apoptosis and proliferation pathways. In addition, it is also required for mouse embryo development. To fully understand the biological functions of caspase-8 in vivo, we screened caspase-8 interacting proteins from mouse T-cell lymphoma and 7. 5 day embryo cDNA libraries by yeast two-hybrid system and got eighteen positive clones, and co-immunoprecipitation (co-IP) experiments validated that one of these proteins called ACT9, interacted with caspase-8 in human 293T cells.

机译：Caspase-8也称为Flice，参与了真核细胞的凋亡过程。最近的研究表明，Caspase-8涉及细胞凋亡和增殖途径。此外，对小鼠胚胎发育也需要它。为了充分了解Caspase-8在体内Caspase-8的生物学功能，我们通过酵母双杂交系统筛选来自小鼠T细胞淋巴瘤的Caspase-8交互蛋白和7. 5天胚胎cDNA文库并获得18个阳性克隆和共免疫沉淀（CO-IP）实验验证了这些蛋白质中的一种，称为ACT9，与人293T细胞中的Caspase-8相互作用。

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《International symposium on medical and pharmaceutical biotechnology》|2009年||共3页
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中图分类生物医学工程;
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caspase-8; yeast two-hybrid; co-immunoprecipitation;

机译：Caspase-8;酵母双杂交;共免疫沉淀;

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2. Screening of the proteins interacting with NS1 of TMUV by yeast two-hybrid system and the identification of the function of the interacted protein [J] . Wang Zhenzhong, Yu Xianglong, Niu Xiaoyu, Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases . 2018,第期

机译：筛选酵母双杂交系统与TMUV NS1相互作用的筛选及鉴定互动蛋白的函数
3. Screening of genes for proteins interacting with the PS1TP5 protein of hepatitis B virus: probing a human leukocyte cDNA library using the yeast two-hybrid system [J] . ZHANG Jian-kang, ZHAO Long-feng, CHENG Jun, Chinese Medical Journal . 2006,第22期

机译：与乙型肝炎病毒PS1TP5蛋白质相互作用的蛋白质的基因筛选：使用酵母双杂交系统探查人类白细胞cDNA文库
4. Yeast two-hybrid system based screening of caspase-8 interacting proteins, and co-immunoprecipitation validation [C] . Wang Lu, Pan Xiao, Hua Zi-Chun International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会) . 2009

机译：基于酵母双杂交系统的caspase-8相互作用蛋白的筛选和共免疫沉淀验证
5. Screening of human pancreatic cDNA expression library for interacting sequences with normal and oncogenic Rasp21 using yeast two-hybrid analysis. [D] . Pandya, Manaswi V. 2009

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6. Screening of genes of proteins interacting with p7 protein of hepatitis C virus from human liver cDNA library by yeast two-hybrid system [O] . Yan-Ping Huang, Shu-Lin Zhang, Jun Cheng, 2005

机译：酵母双杂交系统从人肝cDNA文库中筛选与丙型肝炎病毒p7蛋白相互作用的蛋白基因
7. Screening interacting proteins of clock protein KaiC from Microcystis aeruginosa by the yeast two-hybrid system [O] . 王靖 2008

机译：酵母双杂交系统从铜绿微囊藻中筛选时钟蛋白KaiC的相互作用蛋白

Yeast two-hybrid system based screening of caspase-8 interacting proteins, and co-immunoprecipitation validation

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