Establishment of Stable Focal adhesion kinase(FAK) RNA Interference Cell Line

机译：建立稳定焦粘连激酶（FAK）RNA干扰细胞系

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Malignant melanoma continues to remain a significant health threat, with death often occurring as a result of metastasis. Focal adhesion kinase (FAK) is a non-receptor protein tyrosine kinase implicated in cell cycle progression and cell migration. Overexpression of FAK in a variety of tumors has suggested that FAK is a promising target for therapeutic intervention. In our previous study, we have demonstrated that plasmid-encoded FAK small interfering RNA (siRNA) dramatically inhibited in vitro mouse melanoma cell line B16F10 proliferation and invasion. Here, to further investigated the functional role of FAK, we repressed FAK expression by stable transfection of plasmid-encoded FAK small interfering RNA (siRNA). This stable cell line will be used to investigate the molecular mechanisms that promote an aggressive phenotype of melanoma.

机译：恶性黑素瘤继续仍然是一个重要的健康威胁，死亡经常由于转移而发生。局灶性粘附激酶（FAK）是涉及细胞周期进展和细胞迁移的非受体蛋白酪氨酸激酶。在各种肿瘤中的FAK过度表达表明FAK是治疗干预的有希望的目标。在我们以前的研究中，我们已经证明了质粒编码的FAK小干扰RNA（siRNA）显着抑制体外小鼠黑色素瘤细胞系B16F10增殖和侵袭。在这里，为了进一步研究FAK的功能作用，通过稳定转染质粒编码的FAK小干扰RNA（siRNA）来抑制FAK表达。该稳定的细胞系将用于研究促进黑素瘤的侵袭性表型的分子机制。

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《International symposium on medical and pharmaceutical biotechnology》|2009年||共3页
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中图分类生物医学工程;
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FAK; stable transfection; B16F10; siRNA;

机译：FAK;稳定转染;B16F10;siRNA;

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1. The residence time of focal adhesion kinase (FAK) and paxillin at focal adhesions in renal epithelial cells is determined by adhesion size, strength and life cycle status [J] . LeDévédecS.E., GevertsB., deBontH., Journal of Cell Science . 2012,第19期

机译：黏着斑大小，强度和生命周期状态决定了黏着斑激酶（FAK）和Paxillin在黏着斑在肾上皮细胞中的停留时间
2. RNA interference elucidates the role of focal adhesion kinase in HLA class I-mediated focal adhesion complex formation and proliferation in human endothelial cells. [J] . Jin YP, Korin Y, Zhang X, The Journal of Immunology: Official Journal of the American Association of Immunologists . 2007,第12期

机译：RNA干扰阐明了黏着斑激酶在人类内皮细胞中HLA I类介导的黏着斑复合物形成和增殖中的作用。
3. RNA interference elucidates the role of focal adhesion kinase in HLA class I-mediated focal adhesion complex formation and proliferation in human endothelial cells. [J] . Jin YP, Korin Y, Zhang X, The Journal of Immunology: Official Journal of the American Association of Immunologists . 2007,第12期

机译：RNA干扰阐明了黏着斑激酶在人类内皮细胞中HLA I类介导的黏着斑复合物形成和增殖中的作用。
4. Establishment of Stable Focal adhesion kinase(FAK) RNA Interference Cell Line [C] . Lan Yan, Tang Bo, Hua Zi-Chun International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会) . 2009

机译：稳定的黏着斑激酶（FAK）RNA干扰细胞系的建立
5. Necroptosis and Its Interactions with Focal Adhesion Kinase (FAK) and Extracellular Receptor Kinase (ERK1/2) Following Optic Nerve Injury [D] . D'Onofrio, Philippe Matteo. 2019

机译：椎间盘神经损伤后骨髓凋亡及其与局灶性粘附激酶（FAK）和细胞外受体激酶（ERK1 / 2）的相互作用
6. The direct effect of Focal Adhesion Kinase (FAK) dominant-negative FAK FAK-CD and FAK siRNA on gene expression and human MCF-7 breast cancer cell tumorigenesis [O] . Vita M Golubovskaya, Min Zheng, Li Zhang, 2009

机译：黏着斑激酶（FAK）显性阴性FAKFAK-CD和FAK siRNA对基因表达和人MCF-7乳腺癌细胞肿瘤发生的直接影响
7. The direct effect of Focal Adhesion Kinase (FAK), dominant-negative FAK, FAK-CD and FAK siRNA on gene expression and human MCF-7 breast cancer cell tumorigenesis [O] . Zhang Li, Zheng Min, Golubovskaya Vita M, 2009

机译：黏着斑激酶（FAK），显性阴性FAK，FAK-CD和FAK siRNA对基因表达和人MCF-7乳腺癌细胞肿瘤发生的直接影响

Establishment of Stable Focal adhesion kinase(FAK) RNA Interference Cell Line

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