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Immobilization of BSA on The Polymethacrylic Acid

机译:将BSA固定在聚甲基丙烯酸上

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N,N'-Methylenebisacrylamide was used as the crosslinking agent to prepare the polymethacrylic acid and its carboxyl groups were modified by N- (3- Dimethy--laminopropyl)-N' -ethyl carbodiimide hydrochloride and N-Hydroxy succinimide. The ability of immobilizing BSA and the influence of reacting time of carrier were studied. The immobilization amount and the optimal reacting time were 2100mg/g and 90 minutes respectively. The carrier was characterized by infrared detection. The results indicated that the polymethacrylicacid possessed good performance and the ability to immobilized BSA was enhanced after the modification of EDC-NHS.
机译:N,使用N'-二甲基双丙烯酰胺作为交联剂,制备聚甲基丙烯酸,其羧基通过N-(3-二甲基丙基)-N'-乙基碳二亚胺盐酸盐和N-羟基琥珀酰亚胺改性。研究了生物化BSA的能力和载体反应时间的影响。固定量和最佳反应时间分别为2100mg / g至90分钟。载体的特征在于红外检测。结果表明,在改变EDC-NHS后,聚甲基丙烯酸具有良好的性能和固定的BSA的能力。

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