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首页> 外文会议>ICME International Conference on Complex Medical Engineering >The effects of granulosa cell apoptosis on the developmental competence of bovine oocytes: A study using the well-in-well culture system
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The effects of granulosa cell apoptosis on the developmental competence of bovine oocytes: A study using the well-in-well culture system

机译：甘蓝细胞凋亡对牛卵母细胞发育能力的影响：使用井井培养系统的研究

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In the present study, we succeed to establish the well in the well (WIW) oocyte/embryo culture system. Under this system we studied the effect of granulosa cell apoptosis on oocyte developmental competence. The results show that the hatching rates of blastocysts did not differ (P > 0.05) between oocytes cultured in the WIW system (13%) and those cultured in the group system (16%). In 4-8mm follicles, oocyte developmental competence is higher (P < 0.05) in early atretic follicles (percentage of apoptosis 10-40%) than in the non-atretic follicles (percentage of apoptosis <10%) or in the late atretic follicles (percentage of apoptosis >40%). But in 2-3mm follicles, even though in early atretic follicles the developmental competence of oocyte is also poor (P >0.05). It was indicated that the granulosa cell apoptosis affected the oocyte developmental competence significantly.
机译：在本研究中，我们成功地在井（WiW）卵母细胞/胚胎培养系统中建立良好。在该系统下，我们研究了颗粒细胞细胞凋亡对卵母细胞发育能力的影响。结果表明，在WiW系统（13％）中培养的卵母细胞和组系统中培养的卵母细胞（16％）中的卵母细胞之间的孵化率没有不同（p> 0.05）。在4-8mm的卵泡中，早期贫肠卵泡的卵母发育能力高（P <0.05）（凋亡率为10-40％），而不是在非娱乐卵泡（细胞凋亡百分比<10％）或晚期贫繁卵泡中（细胞凋亡率> 40％）。但在2-3mm的卵泡中，即使在早期的娱乐卵泡中，卵母细胞的发育能力也差（p> 0.05）。结果表明，颗粒细胞凋亡显着影响了卵母细胞发育能力。

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