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The image analysis of ??-fetoprotein expressed in HepG-2 cell clones

机译:HepG-2细胞克隆中表达的胎儿的图像分析

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Objective Using the image analysis methods to research the expression of ??-fetoprotein(AFP) in HepG-2 cell clones. Methods The HepG-2 cells were diluted to make cell clones. And then the expression of AFP in HepG-2 cell clones was detected by immunocytochemistry and analyzed by image analysis system, with HeLa cells as the negative control. Results The expression of AFP was positive in the whole cell clones of HepG-2 by immunocytochemistry staining, while it was negative in HeLa cells. But it was varied in different clones of HepG-2 cells by image analysis. Conclusions The expression of AFP in different clones of HepG-2 cells was varied quantitatively.
机译:目的利用图像分析方法研究Hepg-2细胞克隆中的胎儿蛋白(AFP)的表达。方法稀释HEPG-2细胞以制备细胞克隆。然后通过免疫细胞化学检测HepG-2细胞克隆中AFP的表达,并通过图像分析系统分析,Hela细胞作为阴性对照。结果通过免疫细胞化学染色,AFP的表达在HepG-2的全细胞克隆中,在HeLa细胞中是阴性的。但通过图像分析,它在HepG-2细胞的不同克隆中变化。结论定量变化了HEPG-2细胞不同克隆中AFP的表达。

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