The image analysis of ??-fetoprotein expressed in HepG-2 cell clones

机译：HepG-2细胞克隆中表达的??甲胎蛋白的图像分析

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Objective Using the image analysis methods to research the expression of ??-fetoprotein(AFP) in HepG-2 cell clones. Methods The HepG-2 cells were diluted to make cell clones. And then the expression of AFP in HepG-2 cell clones was detected by immunocytochemistry and analyzed by image analysis system, with HeLa cells as the negative control. Results The expression of AFP was positive in the whole cell clones of HepG-2 by immunocytochemistry staining, while it was negative in HeLa cells. But it was varied in different clones of HepG-2 cells by image analysis. Conclusions The expression of AFP in different clones of HepG-2 cells was varied quantitatively.

机译：目的利用图像分析方法研究β-甲胎蛋白（AFP）在HepG-2细胞克隆中的表达。方法稀释HepG-2细胞，制成细胞克隆。然后以免疫细胞化学法检测AFP在HepG-2细胞克隆中的表达，并通过图像分析系统进行分析，以HeLa细胞为阴性对照。结果通过免疫细胞化学染色，AFP在HepG-2全细胞克隆中呈阳性表达，而在HeLa细胞中呈阴性表达。但是通过图像分析，它在不同的HepG-2细胞克隆中有所不同。结论AFP在HepG-2细胞不同克隆中的表达存在定量变化。

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来源
《4th International Symposium on Bioelectronics and Bioinformatics》|2015年|1-2|共2页
会议地点 Beijing(CN)
作者
Jiang Yang; Wang Qian; Cao Zhenqiang; Du Zhenwu; Zhang Guizhen;
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作者单位

Department of colon and rectum surgery, China-Japan Union Hospital, Jilin University, Changchun, 130033, China;

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原文格式 PDF
正文语种 eng
中图分类
关键词
??-fetoprotein; Cell clones; HepG-2 cells; Image analysis;

机译：甲胎蛋白；细胞克隆； HepG-2细胞；图像分析;

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1. Gene Cloning of Murine α-Fetoprotein Gene and Construction of Its Eukaryotic Expression Vector and Expression in CHO Cells [J] . YI Jlin, TIAN Geng Journal of Huazhong University of Science and Technology . 2003,第4期

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2. Effects of cinobufacini injection on cell proliferation and the expression of topoisomerases in human HepG-2 hepatocellular carcinoma cells [J] . Liu Ying, Ban Li-Ying, Su Xi, Molecular medicine reports . 2015,第1aPta2期

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3. Effects of cinobufacini injection on cell proliferation and the expression of topoisomerases in human HepG-2 hepatocellular carcinoma cells [J] . Liu Ying, Ban Li-Ying, Su Xi, Molecular medicine reports . 2015,第1aPta1期

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4. The image analysis of ??-fetoprotein expressed in HepG-2 cell clones [C] . Jiang Yang, Wang Qian, Cao Zhenqiang, International Symposium on Bioelectronics and Bioinformatics . 2015

机译：HepG-2细胞克隆中表达的胎儿的图像分析
5. Applicability of vaccinia virus as cloning and expression vector for bacterial genes: Mice immune responses to vaccinia virus expressing Brucella abortus and Listeria monocytogenes antigens. [D] . Baloglu, Simge. 2006

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The image analysis of ??-fetoprotein expressed in HepG-2 cell clones

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