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首页> 外文会议>International Conference on Chemical Engineering and Advanced Materials >Immunolocalization and Correlation Frequencies of Lingual Antimicrobial Peptide and Lactoferrin in Bovine Alveolar Epithelium and Bovine Mammary Gland
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Immunolocalization and Correlation Frequencies of Lingual Antimicrobial Peptide and Lactoferrin in Bovine Alveolar Epithelium and Bovine Mammary Gland

机译：牛肺泡上皮和牛乳腺舌抗微生物肽和乳铁蛋白的免疫透明度和相关频率

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Mastitis is most critical disease in dairy cows and causes huge cost in the dairy industry. To prevent and treat it, it is important to understand the mechanisms of immune function in the mammary gland. Innate immunity is non-specific acute-response immune function. Some components of innate immunity in the mammary gland are found, e.g. Lingual Antimicrobial Peptide (LAP), Lactoferrin (LF). These components are found to be localized in the alveolar epithelium of mammary gland. LAP belongs to the p-defensin family, and plays a crucial role in killing a large variety of microorganisms. LF belongs to an iron-binding glycoprotein and has antibacterial activity. It is reported that LF has been localized immunohistochemically in mammary epithelial cells of lactating cows. Our previous study revealed that secretion of LAP into milk proceeded to that of LF after lipopolysaccharide (LPS) injection into the mammary gland. From this result, it is hypothesized that immunohistochemistry probably shows positive to either LF or LAP but not both in the alveolus vs epithelium in the mammary gland. Therefore, the aim of the present study is to investigate the immunolocalization of LAP and LF in the same bovine mammary tissue. Bovine mammary tissues were collected in the slaughterhouse and were fixed with neutralized formalin immediately. Paraffin sections (2-um thickness) were processed with antigen retrieval treatment followed by blocking with casein milk. Sections were cultured with LF antibody or LAP antibody. Immunoreaction products were visualized by incubation with a DAB. LAP and LF were localized in the cytoplasm of epithelial cell of alveolus. In some cases, LAP and LF were seen clearly in the same alveoli of section. In other cases, some epithelial cells were stained only LAP, but not LF, and other epithelial cells of alveolus were stained only LF, but not LAP. These results suggest the possibility that LAP and LF are differentially synthesized in the alveolar epithelium and may support our previous findings that their secretion occurs at the different time course.
机译：乳腺炎是奶牛最关键的疾病，并在乳制品行业导致巨大的成本。为了预防和治疗，重要的是要理解乳腺中免疫功能的机制。先天免疫是非特异性急性反应免疫功能。发现乳腺在乳腺中的原先生免疫组分，例如，语言抗微生物肽（LAP），乳铁蛋白（LF）。发现这些组分在乳腺的肺泡上皮内局部化。 Lap属于P-Defensin家族，并在杀死各种微生物方面发挥至关重要的作用。 LF属于铁结合糖蛋白并具有抗菌活性。据报道，LF已在哺乳动物上皮细胞中局部免疫组织化学。我们以前的研究表明，在脂多糖（LPS）注射到乳腺后，将液体分泌到LF后的LF。从该结果中，假设免疫组织化学可能显示为LF或膝部的阳性，但在乳腺中的肺泡上的肺泡上。因此，本研究的目的是研究在同一牛乳腺组织中的圈子和LF的免疫悬垂性。在屠宰场收集牛乳腺组织，并立即用中和福尔马林固定。用抗原检索处理加工石蜡切片（2-UM厚度），然后用酪蛋白乳封闭。用LF抗体或LAP抗体培养部分。通过与DAB孵育来可视化免疫反应产品。 LAP和LF局部化在肺泡上皮细胞的细胞质中。在某些情况下，在相同的牙髓中清楚地看到leap和lf。在其他情况下，一些上皮细胞仅染色，但不是LF，并且肺泡的其他上皮细胞仅染色LF，但不圈。这些结果表明LAP和LF在肺泡上皮中差异合成的可能性，并且可以支持我们以前的发现，它们的分泌发生在不同的时间过程中。

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