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首页> 外文会议>International Conference on Chemical Engineering and Advanced Materials >Purification of ferulic acid, senkyunolide I and senkyunoiide H from the volatile oil of Rhizoma Chuanxiong using high-speed countercurrent chromatography and preparative liquid chromatography
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Purification of ferulic acid, senkyunolide I and senkyunoiide H from the volatile oil of Rhizoma Chuanxiong using high-speed countercurrent chromatography and preparative liquid chromatography

机译：使用高速逆流色谱法和制备液相色谱法从Rhizoma Chuanxiong挥发油中纯化阿魏酸，Senkyunolide I和SenkyunoIide H.

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摘要

A high-speed countercurrent chromatography and preparative liquid chromatography method was successfully applied to the separation and purification of ferulic acid, senkyunolide I and senkyunolide H from the volatile oil of the traditional Chinese medicinal plant Rhizoma Chuanxiong. For the first time, using a two-phase solvent system composed of N-butanol-acetic acid-water (4:1:5, v/v) and a flow rate of 2 mL?min~(-1), a mixture of SEI and SEH was rapidly obtained by high-speed countercurrent chromatography (HSCCC). The mixture was separated by pre-HPLC with a methanol-water (50:50) mobile phase and flow rate of 5 mL-min"1. The purities of the separated components as determined by high-performance liquid chromatography were 93%, and the combination of ESI-MS and NMR analyses confirmed the chemical structures of the three compounds.
机译：成功地应用了高速逆流色谱和制备液相色谱法，从中药植物Rhuanomag的挥发油中成功地应用于阿魏酸，Senkyunolide I和Senkyunolide H的分离和纯化。首次使用由正丁醇 - 乙酸 - 水（4：1：5，v / v）组成的两相溶剂系统，流速为2mlΩmin〜（-1），混合物通过高速逆流色谱（HSCCC）迅速获得SEI和SEH。用甲醇 - 水（50:50）流动相和5mL-min的流速分离混合物。由高效液相色谱法测定的分离组分的纯度为93％， ESI-MS和NMR分析的组合证实了三种化合物的化学结构。

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