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首页> 外文会议>International Conference on Remote Sensing, Environment and Transportation Engineering >Simultaneous Determination of Asiaticoside And Its Novel Metabolites In Culture of Aspergillus niger by SPE-HPLC Determination of Asiaticoside and its Novel metabolites
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Simultaneous Determination of Asiaticoside And Its Novel Metabolites In Culture of Aspergillus niger by SPE-HPLC Determination of Asiaticoside and its Novel metabolites

机译：孢子蛋白酶苷法测定Asiaticoside及其新代代谢物的曲霉患者Asiaticoside及其新代谢物的同时测定Asiaticoside及其新代谢物

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摘要

When cultured with Aspergillus niger, asiaticoside, purified from Centella Asiatica (L.) Urban, was metabolized into four metabolites including one novel alkaloid. A simple and rapid SPE-HPLC method for simultaneous determination of asiaticoside and its four metabolites in culture of A. niger was developed. The chromatographic separation was achieved on a Dikma Diamonsi C_(18) ODS column (200×4.6 mm i.d.) by gradient elution with 0.1% phosphoric acid in water and 0.1% phosphoric acid in methanol as the gradient mixtures. The flow rate was 1 mL/min, the detection wavelength was 204 nm and the column temperature was kept at 28°C. The retention times of asiaticoside, asiatic acid, M_1, M_2, and M_3 were 11.3, 9.4 and 17.1, 19.3 and 7.9 min, respectively. The mean recoveries of five analysts were all over 99%. Quantification limits were 0.02 μg/mL for all tested compounds. The method was sucessively applied to the quantification of the biotransformation of asiaticoside.
机译：当用曲霉培养培养时，从Centella Asiatica（L.）城市纯化的Asiaticoside，被代谢成四种代谢物，包括一种新的生物碱。开发了一种简单且快速的SPE-HPLC方法，用于同时测定AsiaticoIside及其培养中的四种代谢物。通过在水中的0.1％磷酸和甲醇中的0.1％磷酸在水中的梯度洗脱，在甲醇中的梯度洗脱和0.1％磷酸作为梯度混合物，在Dikma Diamonsi C_（18）ODS柱（200×4.6mm I.D.）上进行色谱分离。流速为1ml / min，检测波长为204nm，柱温保持在28℃。 Asiaticoside，Asiatic acid，M_1，M_2和M_3的保留时间分别为11.3,9.4和17.1,19.3和7.9分钟。五个分析师的平均恢复均超过99％。所有测试化合物的定量限制为0.02μg/ ml。该方法被成功地应用于Asiaticoside的生物转化的定量。

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